MmuEX0013394 @ mm10
Exon Skipping
Gene
ENSMUSG00000024292 | Cyp4f14
Description
cytochrome P450, family 4, subfamily f, polypeptide 14 [Source:MGI Symbol;Acc:MGI:1927669]
Coordinates
chr17:32911655-32917087:-
Coord C1 exon
chr17:32916688-32917087
Coord A exon
chr17:32914538-32914682
Coord C2 exon
chr17:32911655-32911708
Length
145 bp
Sequences
Splice sites
3' ss Seq
TGTCCCTGTATTGTTTGCAGGTA
3' ss Score
10.68
5' ss Seq
CAGGTACCC
5' ss Score
8.63
Exon sequences
Seq C1 exon
TGCTTTGCTAGCTCTGGTTATTCCCCTCACCAGCATACCCCTCCTCCCCCTATTCATCTTCTTCTCCACTCCTGTACCTCTTCACCCAACATTTCCTTGGTTCTCAAGTCTCCTTTCTGCTCTATCCTCTGTCCAGTGTCTTGAGATTATTGTCCCTTTCCGGGACCTCAGTTACTCACTGCACATTTATTCACCCAGCAGGATGTCACAGCTGAGCCTGTCCTGGTTGGGACTGGGGCCCGAGGTGGCCTTCCCATGGAAGACCCTGCTACTGCTTGGGGCCTCCTGGATCCTGGCCCGGATTCTGATCCAGATCTATGCTGCCTATAGGAACTATCGTCACCTCCATGGCTTCCCTCAGCCCCCCAAACGGAACTGGCTTATGGGTCACGTGGGCATG
Seq A exon
GTAACTCCTACAGAGCAGGGCTTGAAGGAATTGACTCGTTTGGTGGGTACCTACCCCCAAGGTTTTTTGATGTGGATTGGACCAATGGTCCCTGTTATTACTCTATGCCATTCTGACATCGTCCGATCTATCCTCAATGCCTCAG
Seq C2 exon
CCGCTGTTGCACTCAAGGATGTGATATTCTACAGCATCCTAAAGCCTTGGCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024292_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=PU(3.0=21.2)
A:
PF0006717=p450=FE(10.3=100)
C2:
PF0006717=p450=FE(10.3=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGTCACAGCTGAGCCTGTC
R:
GCCAAGGCTTTAGGATGCTGT
Band lengths:
250-395
Functional annotations
There are 3 annotated functions for this event
PMID: 10409674
Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B(4) by omega-oxidation in human neutrophils. The CYP4F3 gene contains 14 exons and 13 introns. The cDNAs for CYP4F3A (the neutrophil isoform) and CYP4F3B have identical coding regions, except that they contain exons 4 (HsaEX0002240) and 3 (HsaEX0002239), respectively. Both exons code for amino acids 66-114 but share only 27% identity. When expressed in COS-7 cells, the K(m) of CYP4F3B was determined to be 26-fold higher than the K(m) of CYP4F3A using leukotriene B(4) as a substrate.
PMID: 11461919
Substrate specificity. Mutually exclusive exons, each coding for 48 amino acids, generate isoforms of human CYP4F3 that differ in substrate specificity, tissue distribution, and biological function. CYP4F3B (including HsaEX0002239) has a 30-fold higher Km for LTB4 compared with CYP4F3A, but it utilizes arachidonic acid as a substrate for omega-hydroxylation (Km = 22 microm) and generates 20-HETE, an activator of protein kinase C and Ca2+/calmodulin-dependent kinase II.
PMID: 15145985
Among recombinant P450 enzymes, CYP4F2 and CYP4F3B (uses mutually exclusive exon HsaEX0002239) catalyzed mainly the omega-hydroxylation of C18-epoxides with an apparent Vmax of between 0.84 and 15.0 min_1, whereas the apparent Vmax displayed by CYP4F3A, the isoform found in leukocytes (uses mutually exclusive exon HsaEX0002240), ranged from 3.0 to 21.2 min_1.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types