DreEX0025514 @ danRer10
Exon Skipping
Gene
ENSDARG00000053530 | cyp4f3
Description
cytochrome P450, family 4, subfamily F, polypeptide 3 [Source:ZFIN;Acc:ZDB-GENE-070410-108]
Coordinates
chr12:48580947-48588738:-
Coord C1 exon
chr12:48588586-48588738
Coord A exon
chr12:48581621-48581765
Coord C2 exon
chr12:48580947-48581000
Length
145 bp
Sequences
Splice sites
3' ss Seq
TGTTTATCTGCGTGCTTCAGATG
3' ss Score
6.97
5' ss Seq
CAGGTGGAC
5' ss Score
3.09
Exon sequences
Seq C1 exon
ATGCTGTTTGGACTCTCGGTAACCGGGATCCTGGCGCTCTCCGTTGCCGCGCTTCTTTGCGCGCTCGTGACGCGGATCATTATTAACCGGCGCGCGCTCCGGTGCTTCAACCAGCCGCCGCTCAGGAACTGGATTATGGGCCACATGGGGCTG
Seq A exon
ATGGGTCATAATGAGGAGGGTCTGCAGCGTGTGGATGATCTGGTCTGTAAATACGGTCATTCCTGCTCCTGGTTTCTCGGCCCCTTCTATAACATGGTTCGTCTGTTCCACCCGGACTACATCAGATCCCTCCTGACCGCCTCAG
Seq C2 exon
CTTCCATTACTCTCAAAGACAGGATCTTTTATGGCTTTATGAAGCCCTGGTTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000053530-'0-1,'0-0,1-1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=PU(2.8=25.5)
A:
PF0006717=p450=FE(10.2=100)
C2:
PF0006717=p450=FE(3.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTGTTTGGACTCTCGGTAAC
R:
TCATAAAGCCATAAAAGATCCTGTCT
Band lengths:
194-339
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]