MmuEX0031336 @ mm10
Exon Skipping
Gene
ENSMUSG00000026950 | Neb
Description
nebulin [Source:MGI Symbol;Acc:MGI:97292]
Coordinates
chr2:52149590-52159569:-
Coord C1 exon
chr2:52159477-52159569
Coord A exon
chr2:52150537-52150629
Coord C2 exon
chr2:52149590-52149697
Length
93 bp
Sequences
Splice sites
3' ss Seq
GATACTCTTGGCTCCCCTAGGTA
3' ss Score
8.42
5' ss Seq
TCGGTATTT
5' ss Score
4.52
Exon sequences
Seq C1 exon
GTCAAGTATAAAGAAGCAATCGGACAAGGAACTCCGATCCCTGACCTGCCCGAAGTGAAACGCGTCAAGGAGACCCAGAAGCACATTAGCTCG
Seq A exon
GTATTGTACAAAGAGAACATGAGAAAAGCAACACCAACGCCTGTCACTCCAGACATGGAGAGAGCCAAACGCAACCAAGAAAACATTAGCTCG
Seq C2 exon
GTTCTTTACTCCGATAGCTTCCGGAAACAAATACAAGGGAAAGCTGCCTATGTGCTAGATACACCAGAGATGAGGCGGGTTAGGGAAACCCAACGGCACATCTCAACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026950-'165-185,'165-184,175-185
Average complexity
C1
Mappability confidence:
82%=54=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.848 A=1.000 C2=0.607
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(30.8=25.8),PF0088013=Nebulin=PU(66.7=51.6)
A:
PF0088013=Nebulin=PD(37.9=35.5),PF0088013=Nebulin=PU(55.2=51.6)
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGGAACTCCGATCCCTGACC
R:
TGAGATGTGCCGTTGGGTTTC
Band lengths:
173-266
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types