MmuINT0107221 @ mm10
Intron Retention
Gene
ENSMUSG00000026950 | Neb
Description
nebulin [Source:MGI Symbol;Acc:MGI:97292]
Coordinates
chr2:52149590-52150629:-
Coord C1 exon
chr2:52150537-52150629
Coord A exon
chr2:52149698-52150536
Coord C2 exon
chr2:52149590-52149697
Length
839 bp
Sequences
Splice sites
5' ss Seq
TCGGTATTT
5' ss Score
4.52
3' ss Seq
GACGTGTCTCTCCTCCATAGGTT
3' ss Score
12.3
Exon sequences
Seq C1 exon
GTATTGTACAAAGAGAACATGAGAAAAGCAACACCAACGCCTGTCACTCCAGACATGGAGAGAGCCAAACGCAACCAAGAAAACATTAGCTCG
Seq A exon
GTATTTCCCGATTCAATAGAGCAGTGGAAGACCTTGGAATTAACATTTTAACATCATGAAAACATGCCTTTCTCATTCAACTGAAAACACACTAAAATTTAAAAATAATGGATAATGCAACATCTTGGGAGATTTTTTTTTTAACATCATAAAAATGTGCACTTCTTTTCCTGAAAATGTACACTAAAATTAACACAACTGTGCTTTTTCCATTCCAAACTGTACTAAACATACAATAATCGCCTATGGTGTTCTCCTCTCACATTTGACTCTAAGGTCGTTTCTCATAACCAGCTATATAACAAATTGACCCCCCCCTTAACATCTTCAACCTTCTGCTGTTATTTCTAAGGTACATTATTAAATAGGTAATGCTCTGAAATTTCTGTCTTCACTTTGATATTCTTGTGTCTGGAAGACTCTGACTTTTTTGTTTTGTTTTGGCATATTAGTTTATGTAATCTGGACATTACCTAAGAAAAATGGTCAGTATCAGATTTAATGCAAAAAGTGTCATTAAGTATTCTGGTCTGTATTGCAATTCCTAGTAAATTCAAGAAGACACTTTCTTTCTCTCTTTCAGACTTATACTGTGTCCCTTTGACTTTGAAAATGAAACATTTTCATTTCCAAAGTCAAAGCAAGTCTTGTGTCTTCTGATACAAGTCTTGCCAGTCCTTGTGCTGATCTGGTTCACTAGGTCCAAATATCTGGCTACTGTCTTTTGACTTAAACTAACCTCGGCAGTTACTTCCATTTCAATCTGTTAGTCTTTGAGCTTAAGAATTAGAACAAGGCCATGTATGCCATGTACTCATAGACGTGTCTCTCCTCCATAG
Seq C2 exon
GTTCTTTACTCCGATAGCTTCCGGAAACAAATACAAGGGAAAGCTGCCTATGTGCTAGATACACCAGAGATGAGGCGGGTTAGGGAAACCCAACGGCACATCTCAACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026950:ENSMUST00000075301:146
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.607
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=35.5),PF0088013=Nebulin=PU(55.2=51.6)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGTACAAAGAGAACATGAGAAAAGC
R:
TGTTGAGATGTGCCGTTGGG
Band lengths:
198-1037
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types