Special

MmuEX0035424 @ mm9

Exon Skipping

Gene
ENSMUSG00000057672 | Pkn1
Description
protein kinase N1 [Source:MGI Symbol;Acc:MGI:108022]
Coordinates
chr8:86216535-86223078:-
Coord C1 exon
chr8:86222866-86223078
Coord A exon
chr8:86217859-86218015
Coord C2 exon
chr8:86216535-86216835
Length
157 bp
Sequences
Splice sites
3' ss Seq
AACCAAAAAACTTGCCCTAGAGC
3' ss Score
2.18
5' ss Seq
GAGGTAGGG
5' ss Score
7.81
Exon sequences
Seq C1 exon
GTGACCCACCCTGCAGCCGCTGGGGCCGGAGGGCGCAGCTCGGCGGAGGCCTGGCCAGGCCGGGCCGAGCGCAGCAGGCCGGAGCGCGCCGCGCGCCTGGGAGGGTCTGGGCGCCGCGCCTCCCGCCGCACCCGCCCGCGCGCTCGCAAGCCCTTCCTTGGCGCTGGGACCCCTGGCGGGCGGCCGGAGGACATGGCCGGCGACGCCGTACAG
Seq A exon
AGCTGGCCTGGCGCAGAGGATGCTGGGAACTGGAAGGGAAGAGGAAGTGGCAGTCCAGAGGCCCTGGCAGGGCTGAGGTTCAGGAAGAGGGCGAGGCTCTTAGACCAGACCTACGACCCAGATGGCAGCTGATCCCCCTTTGGATTCGGAGCTGGAG
Seq C2 exon
AGTGAGCCTCGAAGCTGGTCGCTGCTGGAACAGCTGGGTCTGGCTGGGGCTGACCTGGCAGCCCCCGGGGTGCAGCAACAGCTGGAGTTAGAGCGAGAGCGACTGAAGCGGGAGATACGGAAGGAGCTGAAGCTGAAGGAGGGCGCTGAGAACCTGAGGCGAGCCACCACTGACCTGGGCCGTAGCCTGGCCCCTGTGGAGCTGCTGCTGCGGGGCTCCGCTCGACGCCTAGACTTACTGCACCAGCAGCTGCAGGAGCTGCATGCACATGTGGTGCTGCCTGACCCTGCAGCTGGAAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057672-'0-3,'0-1,2-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (No Ref, Alt. ATG)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.529 A=0.723 C2=0.196
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
PF0218511=HR1=WD(100=68.3)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000116235





     
                   









    
Main Skipping Isoform:
ENSMUSP00000005616





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000105443
  



Associated events







Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr19:14550988-14551141 
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr7:12427432-12427584 
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CTCGCAAGCCCTTCCTTGG

				
R: 
TACGGCCCAGGTCAGTGG

				
Band lengths: 
255-412

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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