Special

MmuEX0035429 @ mm9

Exon Skipping

Gene
ENSMUSG00000057672 | Pkn1
Description
protein kinase N1 [Source:MGI Symbol;Acc:MGI:108022]
Coordinates
chr8:86216535-86223078:-
Coord C1 exon
chr8:86222866-86223078
Coord A exon
chr8:86221907-86221971
Coord C2 exon
chr8:86216535-86216835
Length
65 bp
Sequences
Splice sites
3' ss Seq
TCTGTACCCGTCTTCCCCAGTCC
3' ss Score
8.09
5' ss Seq
AAGGTCAGA
5' ss Score
6.8
Exon sequences
Seq C1 exon
GTGACCCACCCTGCAGCCGCTGGGGCCGGAGGGCGCAGCTCGGCGGAGGCCTGGCCAGGCCGGGCCGAGCGCAGCAGGCCGGAGCGCGCCGCGCGCCTGGGAGGGTCTGGGCGCCGCGCCTCCCGCCGCACCCGCCCGCGCGCTCGCAAGCCCTTCCTTGGCGCTGGGACCCCTGGCGGGCGGCCGGAGGACATGGCCGGCGACGCCGTACAG
Seq A exon
TCCTGCTGATGGCCTGCCGGCCTTGCCGAGGAGCTGGGCGTATCTGGTGCTGCCGATCTCACAAG
Seq C2 exon
AGTGAGCCTCGAAGCTGGTCGCTGCTGGAACAGCTGGGTCTGGCTGGGGCTGACCTGGCAGCCCCCGGGGTGCAGCAACAGCTGGAGTTAGAGCGAGAGCGACTGAAGCGGGAGATACGGAAGGAGCTGAAGCTGAAGGAGGGCGCTGAGAACCTGAGGCGAGCCACCACTGACCTGGGCCGTAGCCTGGCCCCTGTGGAGCTGCTGCTGCGGGGCTCCGCTCGACGCCTAGACTTACTGCACCAGCAGCTGCAGGAGCTGCATGCACATGTGGTGCTGCCTGACCCTGCAGCTGGAAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057672_CASSETTE3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (No Ref, Alt. ATG)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.529 A=0.000 C2=0.196
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
PF0218511=HR1=WD(100=68.3)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000115054





     
                   









    
Main Skipping Isoform:
ENSMUSP00000005616





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000105443
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr19:14434590-14434654 
LOW PSI
HsaEX0047873
(PKN1)
Human
(hg19)
chr19:14545402-14545466 
LOW PSI
HsaEX0047873
(PKN1)
Rat
(rn6)
chr19:24830341-24830405 
ALTERNATIVE
RnoEX0066432
(Pkn1)
Cow
(bosTau6)
chr7:12429785-12429849 
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
chr2R:9271985-9272174 
ALTERNATIVE
DmeEX0005781
(Pkn)




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CTCGCAAGCCCTTCCTTGG

				
R: 
TCAGTCGCTCTCGCTCTAACT

				
Band lengths: 
177-242

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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