MmuEX1028417 @ mm10
Exon Skipping
Gene
ENSMUSG00000022526 | Zfp251
Description
zinc finger protein 251 [Source:MGI Symbol;Acc:MGI:1918841]
Coordinates
chr15:76870282-76871435:-
Coord C1 exon
chr15:76871322-76871435
Coord A exon
chr15:76870653-76870754
Coord C2 exon
chr15:76870282-76870414
Length
102 bp
Sequences
Splice sites
3' ss Seq
GTCTGTTTTCTCTCCCTGAGAGT
3' ss Score
-2.99
5' ss Seq
CCGGTGAGT
5' ss Score
10.9
Exon sequences
Seq C1 exon
GTTCCGCCCCCAGCCGACAGTCAACTTGCCCCTTCTGCGCGGGCCAGGCTGGGTGTAGGTGGGTGCCTGGTCCAGAGGCTGAGGCGAGGCTCCCGCGTTCCCATCGCTCTGCAG
Seq A exon
AGTAAGTTAGGGAGACCCCAGGCCGGGGCTGCCTCCTGCCTACCACGGATTCCAAGGTGGTTCACAGCCCAGATGGCTGACAAGTCCTGCTCCCCGACTCCG
Seq C2 exon
GGTGAGACGCCTCTGACCTTCCAAGATGTGGCTGTGTACTTCTCCAGGGCAGAAGGGCAGCAGCTTAGCCCCCAGGAGCGGGCGCTGTACCGGGATGTGATGTTGGAGAACTATGGGAACGTGGCTTCACTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000022526_MULTIEX1-1/4=C1-2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=0.089
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0135222=KRAB=WD(100=91.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCAGCCGACAGTCAACTTG
R:
GTGAAGCCACGTTCCCATAGT
Band lengths:
236-338
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types