MmuEX6011772 @ mm10
Exon Skipping
Gene
ENSMUSG00000030539 | Sema4b
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:MGI Symbol;Acc:MGI:107559]
Coordinates
chr7:80215624-80216843:+
Coord C1 exon
chr7:80215624-80215722
Coord A exon
chr7:80216329-80216440
Coord C2 exon
chr7:80216730-80216843
Length
112 bp
Sequences
Splice sites
3' ss Seq
ACTTGTCTACTCCTCCACAGCAC
3' ss Score
8.11
5' ss Seq
TTGGTAAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
CGTGACTGTCAAAACTACATCAAGATCCTCCTGCCACTCAACAGCAGCCACCTGCTCACCTGTGGCACGGCCGCCTTCAGCCCCCTGTGTGCTTACATT
Seq A exon
CACATAGCGAGCTTTACTTTAGCCCAAGATGAGGCCGGCAATGTCATTCTGGAGGATGGCAAGGGTCGTTGTCCCTTTGACCCCAACTTCAAGTCCACGGCTCTGGTGGTTG
Seq C2 exon
ATGGTGAGCTGTACACTGGAACAGTCAGTAGCTTCCAGGGAAACGACCCAGCCATTTCCCGGAGCCAGAGTTCCCGCCCCACCAAGACTGAGAGCTCCCTCAACTGGCTACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030539-'14-12,'14-11,16-12
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.333
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(30.5=100)
A:
PF0140314=Sema=FE(8.4=100)
C2:
PF0140314=Sema=FE(8.7=100)
Main Skipping Isoform:
ENSMUST00000032754fB16432
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGTGACTGTCAAAACTACATCAAGA
R:
TTGTAGCCAGTTGAGGGAGCT
Band lengths:
212-324
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types