MmuEX6011774 @ mm9
Exon Skipping
Gene
ENSMUSG00000030539 | Sema4b
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:MGI Symbol;Acc:MGI:107559]
Coordinates
chr7:87343405-87358211:+
Coord C1 exon
chr7:87343405-87343656
Coord A exon
chr7:87357682-87357845
Coord C2 exon
chr7:87358149-87358211
Length
164 bp
Sequences
Splice sites
3' ss Seq
ATGCTGACTTCTCTCCCCAGGCT
3' ss Score
9.61
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
Exon sequences
Seq C1 exon
GTCCAGAGGCGGAGGCCGAGGGAAGGACTCCGGGCCGAACCGCGGGGCGGGGCTCCAGCCTGCGATTCCACTTGAGCAACCTGAGCCGGAGGAGGACGCCGCTGCCTGCTGCGGTCCGAGCTCCACGCGCCGCGATGGGCCGGGCGAGCCGCTCCGCTGTCCTGCGGCGCGCGCTGCTGCTGCTGCTGCTACTGCTGCTGCTGCGGACGACGACTACGCGGGCGCTCGGCCCCCGGATCAGTGTGCCGCTGG
Seq A exon
GCTCTGAAGAAAGGCTGATTAGAAAATTTGAAGCTGAAAACATCTCCAACTACACGGCCCTTCTGCTGAGCCAGGATGGCAAGACGCTGTATGTGGGGGCCCGAGAGGCCCTCTTTGCACTTAACAGCAACCTCAGCTTCTTGCCAGGCGGGGAGTACCAAGAG
Seq C2 exon
CTGCTGTGGAGTGCAGATGCTGACAGGAAGCAGCAGTGCAGCTTCAAGGGCAAGGACCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030539-'3-3,'3-2,4-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0140314=Sema=PU(8.4=67.3)
C2:
PF0140314=Sema=FE(4.6=100)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGCGATTCCACTTGAGCAA
R:
CTTGCCCTTGAAGCTGCACT
Band lengths:
248-412
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: