RnoEX0079310 @ rn6
Exon Skipping
Gene
ENSRNOG00000025167 | Sema4b
Description
semaphorin 4B [Source:RGD Symbol;Acc:1307872]
Coordinates
chr1:141986145-142001124:+
Coord C1 exon
chr1:141986145-141986322
Coord A exon
chr1:142000627-142000790
Coord C2 exon
chr1:142001062-142001124
Length
164 bp
Sequences
Splice sites
3' ss Seq
GTCCGATTTTCTCTCCTCAGACT
3' ss Score
9.33
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
Exon sequences
Seq C1 exon
ACTCCACTTGAGCAACCTGAGCCGGAGGACGCCGCTCCTGCCGCGGTCCGAGCTCCACGCGCCGCGATGGGCCGGGCGAGCCGCTCCGCCGACCTGCTGAGCGCGCTGCAGCTGCTGTTGCTACTGCTGCGGACGACGACGGCGCGGGCGCTCGGCCCACGGATCAGTGTGCCGCTGG
Seq A exon
ACTCTGAAGAAAGGCTGATTAGAAAATTTGAAGCTGAAAACATCTCCAACTACACGGCCCTTCTGCTGAGCCAAGATGGCAAGACGCTGTATGTGGGGGCCCGAGAGGCCCTCTTTGCCCTTAACAGCAACCTCAGCTTCTTGCCGGGCGGGGAGTACCAAGAG
Seq C2 exon
CTGCTGTGGAGTGCAGATCCTGACAGGAAGCAGCAGTGCAGCTTCAAGGGCAAGGACCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025167-'0-4,'0-3,4-4
Average complexity
S
Mappability confidence:
88%=75=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.190
Domain overlap (PFAM):
C1:
NO
A:
PF0140314=Sema=PU(8.4=67.3)
C2:
PF0140314=Sema=FE(4.6=100)
Main Skipping Isoform:
ENSRNOT00000030914fB13114
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCCACTTGAGCAACCTGAGC
R:
GGTCCTTGCCCTTGAAGCTG
Band lengths:
235-399
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]