Special

MmuEX6086257 @ mm9

Exon Skipping

Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66907169-66908583:+
Coord C1 exon
chr11:66907169-66907259
Coord A exon
chr11:66907976-66908365
Coord C2 exon
chr11:66908457-66908583
Length
390 bp
Sequences
Splice sites
3' ss Seq
GGCATCCCCGCTCTCTTTAGGCT
3' ss Score
8.13
5' ss Seq
AAGGTGAGG
5' ss Score
9.16
Exon sequences
Seq C1 exon
GAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGATGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAG
Seq A exon
GCTCGCATCGAGGAGCTGGAGGAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGCTGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAAGCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTGGCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGAGAGCGTGTCCAAATCCAAG
Seq C2 exon
GCCAATCTGGAGAAGATCTGCCGAACTCTAGAGGATCAGTTAAGTGAGGCCAGGGGCAAGAATGAGGAGATGCAGAGGAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAGAGGCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-'29-29,'29-28,30-29=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.415 C2=0.698
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0003816=Filament=FE(11.0=100),PF0157614=Myosin_tail_1=FE(3.5=100)

							
A:
PF0003816=Filament=FE(47.4=100),PF0157614=Myosin_tail_1=FE(15.0=100),PF104734=CENP-F_leu_zip=PU(41.5=45.4)

							
C2:
PF0003816=Filament=FE(15.4=100),PF0157614=Myosin_tail_1=FE(4.9=100),PF104734=CENP-F_leu_zip=FE(29.6=100),PF059116=DUF869=PU(39.0=90.7)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000007301





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000104329, ENSMUSP00000131883
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr17:10638043-10638432 
HIGH PSI
HsaEX6021092
(MYH3)
Human
(hg19)
chr17:10541360-10541749 
HIGH PSI
HsaEX6021092
(MYH3)
Rat
(rn6)
chr10:53637920-53638309 
Cow
(bosTau6)
chr19:30236742-30237131 
HIGH PSI
BtaEX6087220
(MYHC-EMBRYONIC)
Chicken
(galGal4)
chr18:544624-545013 
Chicken
(galGal3)
chr18:598297-598686 
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GAAGGACTTTGAATATAGTCAGCTGC

				
R: 
CTCTGTCTGCAGCCGGGA

				
Band lengths: 
214-604

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]










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