Special

MmuINT0010272 @ mm9

Intron Retention

Gene
Description
acyl-CoA synthetase medium-chain family member 4 [Source:MGI Symbol;Acc:MGI:2681844]
Coordinates
chr7:126854616-126854941:+
Coord C1 exon
chr7:126854616-126854743
Coord A exon
chr7:126854744-126854841
Coord C2 exon
chr7:126854842-126854941
Length
98 bp
Sequences
Splice sites
5' ss Seq
TGGGTTTGT
5' ss Score
0.77
3' ss Seq
AGGTTTTCTACCCTCTGCAGGTA
3' ss Score
8.9
Exon sequences
Seq C1 exon
GACAATCCAGAAAAGACAGATGCCACAATAAGAAGAAATTTTTATGTCACTGGAGACAGAGGAGTGATGGATGATGACGGATACTTTTGGTTTGTCGGCAGAGCTGATGATGTCATTATATCTTCTGG
Seq A exon
GTTTGTATGTTTGCTGTTCTAAGAAACTGATGACATGACAGAATGGAAGATTTCCAATTTGGAGAGTGTGATGATCAAAGGTTTTCTACCCTCTGCAG
Seq C2 exon
GTACCGAATTGGGCCTTTTGAAGTAGAGAGCGCACTGATAGAGCACCCAGCAGTAGTTGAGTCAGCTGTTGTCAGCAGTCCAGATCCGATCCGAGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000047026-Acsm4:NM_178414:11
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.023 A=NA C2=0.000
Domain overlap (PFAM):

C1:
PF0050123=AMP-binding=PD(9.9=95.3)
A:
NA
C2:
PF131931=AMP-binding_C=PU(32.1=76.5)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Human
(hg38)
No conservation detected
Rat
(rn6)
No conservation detected
Zebrafish
(danRer10)
ALTERNATIVE
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAGACAGAGGAGTGATGGA
R:
ATCGGATCTGGACTGCTGACA
Band lengths:
168-266
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types