MmuINT0025665 @ mm10
Intron Retention
Gene
ENSMUSG00000026918 | Brd3
Description
bromodomain containing 3 [Source:MGI Symbol;Acc:MGI:1914632]
Coordinates
chr2:27463455-27464193:-
Coord C1 exon
chr2:27463889-27464193
Coord A exon
chr2:27463593-27463888
Coord C2 exon
chr2:27463455-27463592
Length
296 bp
Sequences
Splice sites
5' ss Seq
CCTGTGAGT
5' ss Score
7.21
3' ss Seq
TCTGAACATCTCTTTCCTAGGAT
3' ss Score
7.93
Exon sequences
Seq C1 exon
GTGCCTGATTGGGCCCAGAAATGGGATGCCAAGCCTCCACGAGGAGCGCCTCTTTGAGCTGCTGCTGTGACAGCAAGTGACTAAGCTGCCAGAGGATGTCCACTACAGCGGCTGCCCCCACGGGGATCCCGGCAGTCCCGGGCCCCGTGAACCCTCCCCCACCTGAGGTCTCCAACCCCAGCAAGCCCGGGCGAAAGACTAACCAACTGCAGTACATGCAGAATGTAGTGGTGAAGACACTCTGGAAACATCAGTTTGCCTGGCCTTTCTACCAGCCTGTGGATGCAATCAAGCTGAACCTGCCT
Seq A exon
GTGAGTGGCCCTTAGTGTCTGAGATATAGCAGGGGCTGCCCGTGCCTCAGAAGCCAGTGGTCTGGCAAGGTGGTTGCCCCTAGTACAAGAAATGGGGAGAACCTACAGCCTCCATGGTGAAAGAATACAAGGAAAGGGCTAGAGCAGCGGGTCTCAGGAGACTCTCCTCAGACCACATGGCCCTCCTGCTCTCAGGCCTCTGGGACCCAGCTGATGTGGGAGGACTTTGCTGTTCTTGGGAAGTGTGAAAATAACATTGGCTATATGAGTAACACTTCTGAACATCTCTTTCCTAG
Seq C2 exon
GATTATCATAAAATAATAAAAAACCCAATGGACATGGGGACTATCAAGAAGAGACTAGAAAATAATTATTATTGGAGTGCCAGTGAGTGTATGCAGGACTTCAACACCATGTTTACAAACTGTTATATTTATAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026918:ENSMUST00000028282:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.442 A=NA C2=0.004
Domain overlap (PFAM):
C1:
PF0043920=Bromodomain=PU(33.3=41.4)
A:
NA
C2:
PF0043920=Bromodomain=FE(51.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGTCCACTACAGCGGCTG
R:
GCATACACTCACTGGCACTCC
Band lengths:
305-601
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types