MmuINT0028785 @ mm10
Intron Retention
Gene
ENSMUSG00000035547 | Capn5
Description
calpain 5 [Source:MGI Symbol;Acc:MGI:1100859]
Coordinates
chr7:98129244-98131268:-
Coord C1 exon
chr7:98131191-98131268
Coord A exon
chr7:98129440-98131190
Coord C2 exon
chr7:98129244-98129439
Length
1751 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
3' ss Seq
CATTGCCTTCCTGCACTTAGGAT
3' ss Score
9.18
Exon sequences
Seq C1 exon
GTCAGAGGAATGGCAGAAAGTGAGCAAGAGTGAGAGGGAGAAGATGGGCGTGACCGTGCAGGATGATGGGGAATTCTG
Seq A exon
GTGAGTGTCTTCTTGTCTTGAGTTTGGGCTGGGGAGGACCTTCCTGGGGCCTGGCAGGAGCACAGGCCAGGGAGGCAGGAGCCAGGGCCACAGTCATCAGTTTGCTATATAGCCTTGGAAACATCACATTACCCCTCTGATCCGTTGCCACCTCTGTAAATGCGTTGTATAGAGAAGGGTGCTTTTACCCCATGTCCTGTCTGCACACCTGAGGGACACCAGACCTGCCCAGTCACCCAGACCGAGTCTTAGCAAGCAAGACTCAGAGCCAACAGTCAGTCACCTCAGAGCATCATCCCCTGCGTCCGTGCGCTGCAACTGCTGGCTGTGTCCACCCTGCATCCACCCCCAGTGTCCACCCCCTTACATCCCCTTATGACCTGTAGCTATTCCCTACAACACCTCCCATCCATTCCTCCATCTCCATCACCTGTGGTCCACCATTCCTTATATCTTCTTCACACATCTACTCCCTGCATCCGTTTGCTGGACCCGTTCCCTAAATCTATCACCCTGTGCCCATCCCTCGCGTCCAATTTCTCCACACATCCTGTTGCATGTACCCCCTACGCCTACTCACCCCCTTGCTTCTTTCCACTTATTCTTCTGAATGCACCCCTTTCTCTGTTTCCTTACCTATTCATTCTATAAACCCATTTATTTCCCACACCCATCCCCTCATCCAGCCCCTGTACCTTTTCATTCACCCGTATCCATCCCCTCATCCCCTCCCCATACCTATTCATTCTCCTGTATCTATTCCTTCCTTTGTATACACTCCCCTACCTGCATCCCTTGCGCGAATGCATTCCCTTACATCCCTTCCCTCGAATCCATTCGCCAGCATTCTATTTGGACTCCGTTTGGTACCCTGCTCTTCTTCACAGCCCCTACAGGGGAGGGGGGGGACAAGGTGCCTGATGTGAGGTCTATGCCATGCAAACCCACAGGCCAACCGGGAGCAGATGACAGTGACATTAATACCCTTACGAAGCCCCCTTGTGCTGGGGTGGAGGAGGCTGGCCGGGCCCTCCAGTCTGCACTGCTGTGGACAGAGTCCACTTTCTGCAGTCTGGCTCAGGAGTCAGTTGAGTTAAAACATAGCAATGTTGCTCCTCCATGGGAAAAGCTGCTTTTAAAGGCATTTCTCAAGCATATTCAAGCACCTCTGGCACTGCCCATGCTCCTGGAGGTGTGCCTGGCAGCAGCCTGATCACTGCCCTTGCTTACCATGGAGGGAGGGTCAGGGTTGTCTACCTGTTGATTCGGGTCTTACAGCAGCCTCGGAAGACAATGGGTAGAAACAGATCATACATGATGTACATACCAGGGCTGCTGCGAAGGCACTGACCATCAGTCCTGACAACTTCACTTCAATTCCTGGGTCCCACATGGTGGGAGGAGAGGACCTACTTCCACAGGCTGTCCTCTGACCTCCACATGCATGCCCTTCCCCCAGCAATAGTGTTCTAAACTGCAATAAAATGTTAAGATGCACACCCTGGTTATTAACACTGTGAACAGCTGTCACAGCCAACACACCAGCTACACGTACATTCTTGTTTTGCTCAGTGTCTTGCCTTCCCCGTGAGACAGGCAACTGTTGGTAGGTTCATCCCATTTTACAGATGAGGAAGTTGAGGCCCAAGAGAGACTTGGGGTCTTGGTGGCATTGTATCCCGCTTACTTGTGTATAGCTGCGTTTCGGAGCATGGTGCAGTGCACCAAGCCCATTGCCTTCCTGCACTTAG
Seq C2 exon
GATGACCTTTGAGGACATGTGCCGGTACTTTACTGACATCATTAAATGCCGCCTGATTAACACGTCCTACCTGAGCATCCATAAGACATGGGAGGAGGCCCGGCTGCATGGTGCCTGGACGAGACATGAGGACCCACAGCAGAACCGCAGTGGAGGCTGCATCAACCACAAGGACACTTTCTTCCAGAACCCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035547:ENSMUST00000040971:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.343 A=NA C2=0.280
Domain overlap (PFAM):
C1:
PF0064816=Peptidase_C2=FE(8.2=100)
A:
NA
C2:
PF0064816=Peptidase_C2=PD(6.0=28.8),PF0106717=Calpain_III=PU(25.0=54.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGGAATGGCAGAAAGTGAGCA
R:
CTGTGGGTTCTGGAAGAAAGTGT
Band lengths:
270-2021
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types