MmuINT0055813 @ mm10
Intron Retention
Gene
ENSMUSG00000026921 | Egfl7
Description
EGF-like domain 7 [Source:MGI Symbol;Acc:MGI:2449923]
Coordinates
chr2:26589045-26589446:+
Coord C1 exon
chr2:26589045-26589169
Coord A exon
chr2:26589170-26589329
Coord C2 exon
chr2:26589330-26589446
Length
160 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
3' ss Seq
GCTGACCCCATTCCCTGTAGCCG
3' ss Score
5.94
Exon sequences
Seq C1 exon
GCCACAAAAAGAAGAAGGCTACCCCACTTACAGATGCAGACCATGTGGGGCTCCGGAGAACTGCTTGTAGCATGGTTTCTAGTGTTGGCAGCAGATGGTACTACTGAGCATGTCTACAGACCCAG
Seq A exon
GTGAGGAAGGGCTTGGGCCTTGGGGTACTGGGGTCATGACCAGAGGCTCTTAGCTGTGCCCACAGCAGACCCCAATACCTGAGGAGAATTCCCATGTCTGTATGTACCTGGACTGGAAGAGACCTGGCTCTAGACCACTAGCTGACCCCATTCCCTGTAG
Seq C2 exon
CCGTAGAGTGTGTACTGTGGGGATTTCCGGAGGTTCCATCTCGGAGACCTTTGTGCAGCGTGTATACCAGCCTTACCTCACCACTTGCGACGGACACAGAGCCTGCAGCACCTACCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026921:ENSMUST00000166920:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF075468=EMI=PU(0.1=0.0)
A:
NA
C2:
PF075468=EMI=PU(55.7=97.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCACAAAAAGAAGAAGGCTACC
R:
CGGTAGGTGCTGCAGGCT
Band lengths:
242-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types