MmuINT0055823 @ mm10
Intron Retention
Gene
ENSMUSG00000015467 | Egfl8
Description
EGF-like domain 8 [Source:MGI Symbol;Acc:MGI:1932094]
Coordinates
chr17:34614170-34614579:-
Coord C1 exon
chr17:34614484-34614579
Coord A exon
chr17:34614341-34614483
Coord C2 exon
chr17:34614170-34614340
Length
143 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGC
5' ss Score
7.23
3' ss Seq
CTTCTCTTTCTGGTAACTAGATG
3' ss Score
6.05
Exon sequences
Seq C1 exon
CCATCTGCTCCAAGCCTTGTCTTAATGGAGGTGTCTGCACTGGACCAGACCGGTGCGAGTGTGCCCCAGGCTGGGGAGGAAAGCATTGCCACGTGG
Seq A exon
GTGAGCAGGATGGTCCCCCTCCCCCTGTCACACACATACACACACACACATGGGCAATGGGGGTGGGTGCTGAGATGGGGACTGCCCCACTCACCCTGCATCCTGCCTGCCCCCTTGAGCTCTCTTCTCTTTCTGGTAACTAG
Seq C2 exon
ATGTCGATGAATGCAGGGCCAGCCTTACCCTCTGCTCTCATGGCTGCCTCAACACACTGGGCAGCTTCTTGTGCAGCTGTCCACACCCCCTGGTGCTGGGTCTCGATGGACGCACCTGTGCAGGGGGGCCTCCAGAGTCCCCGACAAGTGCCAGCATCCTCAGTGTGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000015467:ENSMUST00000015611:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.115
Domain overlap (PFAM):
C1:
PF0000822=EGF=WD(100=81.8),PF0764510=EGF_CA=PU(0.1=0.0)
A:
NA
C2:
PF0764510=EGF_CA=WD(100=69.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATCTGCTCCAAGCCTTGTCT
R:
CTGAGGATGCTGGCACTTGTC
Band lengths:
258-401
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types