RnoINT0053400 @ rn6
Intron Retention
Gene
ENSRNOG00000000436 | Egfl8
Description
EGF-like-domain, multiple 8 [Source:RGD Symbol;Acc:1549706]
Coordinates
chr20:4378997-4379385:-
Coord C1 exon
chr20:4379290-4379385
Coord A exon
chr20:4379168-4379289
Coord C2 exon
chr20:4378997-4379167
Length
122 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGC
5' ss Score
7.23
3' ss Seq
CTCCTCTTTCTGGTCACTAGATG
3' ss Score
6.93
Exon sequences
Seq C1 exon
CCATCTGCTCTAAGCCTTGTCTTAATGGAGGTGTGTGCGCCGGACCGGACCAGTGCGAGTGTGCCTCAGGCTGGGCAGGGAAGCACTGCCACGTGG
Seq A exon
GTGAGCAGGATAGCTCCGCCCCTCCCCTGCACGGGCAGTGGGGGTGGGTGCTGGGAACGGGATCGCCCCACCCACCCTAGGCATCCTGCCCCCTTCAACTCCCTCCTCTTTCTGGTCACTAG
Seq C2 exon
ATGTGGATGAATGCAGGACCAGCCTTACCCTCTGCTCTCATGGCTGTCTCAACACGCTGGGCAGCTTCACCTGCAGCTGTCCGAACCCCCTGGTGCTGGGGCCCGATGGACGCGCCTGTGCAGGGGGCCCTCCGGAGTCCTCGACGAGTGCTGGCATCCTCAGTGTGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000436:ENSRNOT00000000499:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.129
Domain overlap (PFAM):
C1:
PF079748=EGF_2=PD(90.6=87.9),PF0764510=EGF_CA=PU(0.1=0.0)
A:
NA
C2:
PF0764510=EGF_CA=WD(100=69.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTAATGGAGGTGTGTGCGC
R:
CACTGAGGATGCCAGCACTC
Band lengths:
242-364
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]