Special

MmuINT0103470 @ mm10

Intron Retention

Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67094474-67095081:+
Coord C1 exon
chr11:67094474-67094863
Coord A exon
chr11:67094864-67094954
Coord C2 exon
chr11:67094955-67095081
Length
91 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGG
5' ss Score
9.16
3' ss Seq
CTCCTCTTTCCAACCCGCAGGCC
3' ss Score
10.67
Exon sequences
Seq C1 exon
GCTCGCATCGAGGAGCTGGAGGAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGCTGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAAGCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTGGCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGAGAGCGTGTCCAAATCCAAG
Seq A exon
GTGAGGGAGCGGCTAGGCAGATCCGGAGGGGCAGCTGCAGTGAGTCAACCTAAGGACACCTGCCGACCTCCCTCCTCTTTCCAACCCGCAG
Seq C2 exon
GCCAATCTGGAGAAGATCTGCCGAACTCTAGAGGATCAGTTAAGTGAGGCCAGGGGCAAGAATGAGGAGATGCAGAGGAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAGAGGCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908:ENSMUST00000108689:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.417 A=NA C2=0.698
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0003816=Filament=FE(47.4=100),PF0157614=Myosin_tail_1=FE(39.4=100),PF104734=CENP-F_leu_zip=PU(42.0=44.6)

							
A:
NA

							
C2:
PF0003816=Filament=FE(15.4=100),PF0157614=Myosin_tail_1=FE(12.8=100),PF104734=CENP-F_leu_zip=FE(30.4=100),PF059116=DUF869=PU(35.5=90.7)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000007301





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000104329, ENSMUSP00000131883
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr17:10637936-10638042 
ALTERNATIVE
HsaINT0107738
(MYH3)
Human
(hg19)
chr17:10541253-10541359 
ALTERNATIVE
HsaINT0107738
(MYH3)
Rat
(rn6)
chr10:53638310-53638400 
LOW PSI
RnoINT0096483
(Myh3)
Cow
(bosTau6)
chr19:30236655-30236741 
LOW PSI
BtaINT0097449
(MYHC-EMBRYONIC)
Chicken
(galGal4)
chr18:589707-589869 
Chicken
(galGal3)
chr18:552010-552158 
LOW PSI
GgaINT0127727
([1])
Zebrafish
(danRer10)
chr24:41037019-41037108 
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AGAGCGAGTTCAAGCTGGAGA

				
R: 
GGACTTCTGTGTGGTCAGCTC

				
Band lengths: 
173-264

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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