MmuINT0103524 @ mm9
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67055643-67055877:+
Coord C1 exon
chr11:67055643-67055670
Coord A exon
chr11:67055671-67055762
Coord C2 exon
chr11:67055763-67055877
Length
92 bp
Sequences
Splice sites
5' ss Seq
TACGTAAGT
5' ss Score
9.27
3' ss Seq
ACTTTCATTCTGTCCCTCAGTGG
3' ss Score
8.5
Exon sequences
Seq C1 exon
ATCGTGAGAATCAGTCCATCCTGATTAC
Seq A exon
GTAAGTAGACGCCTCATCTTTCTGTCTGCTGAGTTTCGATTGTTAGGGCAGGACCTCCTGCCTTACCTTCTGACTTTCATTCTGTCCCTCAG
Seq C2 exon
TGGAGAATCCGGGGCCGGGAAGACTGTGAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAGGAGGCGACTTCTGGCAAAATGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-Myh4:NM_010855:5
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.100 A=NA C2=0.179
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(1.3=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGTGAGAATCAGTCCATCCTGA
R:
CTGCATTTTGCCAGAAGTCGC
Band lengths:
142-234
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: