Special

MmuINT0119694 @ mm9

Intron Retention

Gene
Description
phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide [Source:MGI Symbol;Acc:MGI:1203729]
Coordinates
chr7:123485666-123485985:-
Coord C1 exon
chr7:123485876-123485985
Coord A exon
chr7:123485792-123485875
Coord C2 exon
chr7:123485666-123485791
Length
84 bp
Sequences
Splice sites
5' ss Seq
CTTGTGAGT
5' ss Score
7.15
3' ss Seq
GAGACTGCTTTTCTTTATAGGTG
3' ss Score
8.85
Exon sequences
Seq C1 exon
GTGCAGTTCCCTTCAGCCCAACTCTGGGCCAAATAGGAGGAGCAGTGAAGTTATCTGTTTCTTACCGAAATGGCACCCTCTTCATCATGGTGATGCACATCAAAGATCTT
Seq A exon
GTGAGTGGTGACAAATGCTGACTGTAGAGTAAATTTGTACCTCGGTAGGAAGCACCACTATAAAGAGACTGCTTTTCTTTATAG
Seq C2 exon
GTGACTGAAGATGGGGCTGACCCAAATCCCTATGTCAAAACATACCTGCTTCCAGATACCCACAAAACGTCAAAACGTAAAACCAAAATTTCACGTAAAACTAGGAACCCAACATTCAATGAAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030660-Pik3c2a:NM_011083:31
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=NA C2=0.190
Domain overlap (PFAM):

C1:
PF0016825=C2=PU(11.2=27.0)
A:
NA
C2:
PF0016825=C2=FE(46.1=100)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
ALTERNATIVE
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTTCCCTTCAGCCCAACTCT
R:
TGTGGGTATCTGGAAGCAGGT
Band lengths:
170-254
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types