MmuINT0131994 @ mm9
Intron Retention
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136577815-136578297:+
Coord C1 exon
chr5:136577815-136577927
Coord A exon
chr5:136577928-136578104
Coord C2 exon
chr5:136578105-136578297
Length
177 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGG
5' ss Score
7.13
3' ss Seq
TGTTGGTTAATCCCCTGCAGGTG
3' ss Score
8.62
Exon sequences
Seq C1 exon
GTGGCAGGGATGCGCTATCTGCATGGCATCCTGGGCCCCATCATTGACAGAGTGTTCGAGGAGAAGAAGTACGTGGAGCTGGACCCCAGCAAAGTGGAAGTTAAGGATGTAGG
Seq A exon
GTGAGGCCGGGGTAATCGCTGGGTGAACAACTTTGCTACATCAGGTCCTATGTAAAGGTGTTCCTTTTGTCTGGGTTCACACGTCCTTTCTCTCTGTATCTAAAGTCAGATGGGACCCCCAGCTGCCAAGTATTCACAAGTGCTGACCTTGACCGTTTGTTGGTTAATCCCCTGCAG
Seq C2 exon
GTGCTCTGGGCTGCACCGCCCACAGACCGAAGCTGAAGTGTTAGAGCAGAGTGCACAGACGCTGCGTGCCCACTTAGTGGCGCTGCTGAGCGCCATATGCCGCTCGGTTCGCACGTGCCCAGCCATCATCCGCGCCACATTCCGGCAGCTGTTCAGGCGCGTGCGCGAGCGCTTCCCGAACGCCCAGCACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952-Rasa4:NM_133914:12
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.023
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(19.5=100)
A:
NA
C2:
PF0061614=RasGAP=FE(33.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Human
(hg38)
No conservation detected
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTATCTGCATGGCATCCTGG
R:
CTGAACAGCTGCCGGAATGT
Band lengths:
256-433
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: