RnoEX0026031 @ rn6
Exon Skipping
Gene
ENSRNOG00000043513 | Cyp4x1
Description
cytochrome P450, family 4, subfamily x, polypeptide 1 [Source:RGD Symbol;Acc:628719]
Coordinates
chr5:133940927-133942241:-
Coord C1 exon
chr5:133942087-133942241
Coord A exon
chr5:133941411-133941657
Coord C2 exon
chr5:133940927-133941027
Length
247 bp
Sequences
Splice sites
3' ss Seq
GCCCTTTATCCCTGCACTAGGAG
3' ss Score
6.99
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
CACCTATGAGTCTTATGTGAAGGCAACATTTGAACTTGGTGAAATCATATCTTCTCGCTTGTACAATTTCTGGCATCATCATGACATAATTTTCAAACTCAGTCCTAAGGGCCACTGCTTCCAGGAGTTAGGCAAAGTGATACATCAATGCACAG
Seq A exon
GAGGCTGAGGCAGGTGGTTCCTGTGAGCTCGAGGTCAGCCTGGAATTTTGCTGAGTGTTGGGAATCCACATTTTGCTTCCGTGAACATGGATGAGAAGCTAAACCCATGATGTACAGAAAACGTTTTTGAAAATCAAAGCATACTGTCCTACTTACTTCTATCTAAATTCACTTACTGTTTATATGAAACGGTAAATTCAAATAGAAAGCTGACCCCAATTAAGACAGAAAACTAACTGTGTAGAAG
Seq C2 exon
AAAAGATTATCCAGGACAGAAAGAAAACCCTCAAGGATCAAGTAAATCAGGATGACACTCAAACATCTCAAAATTTTCTGGATATTGTTCTTTCTGCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000043513_MULTIEX1-3/6=2-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.088
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(11.4=100)
A:
PF0006717=p450=PD(0.1=0.0)
C2:
PF0006717=p450=FE(7.2=100)
Main Inclusion Isoform:
ENSRNOT00000011985fB3833
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Mouse
(mm9)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACCTATGAGTCTTATGTGAAGGCA
R:
TGGGCAGAAAGAACAATATCCAGA
Band lengths:
255-502
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]