RnoEX0052987 @ rn6
Exon Skipping
Gene
ENSRNOG00000027596 | Mbd5
Description
methyl-CpG binding domain protein 5 [Source:RGD Symbol;Acc:1562961]
Coordinates
chr3:33500925-33503685:+
Coord C1 exon
chr3:33500925-33501000
Coord A exon
chr3:33501261-33501320
Coord C2 exon
chr3:33503558-33503685
Length
60 bp
Sequences
Splice sites
3' ss Seq
ACTTCTGTTTTCTTAAAAAGGTT
3' ss Score
9.39
5' ss Seq
ACTGTGAGT
5' ss Score
7.94
Exon sequences
Seq C1 exon
AAGTGGGAAGCTAAATAACCATTTAGAAGCTGCTATTCACGAGGCCATGAGCGAACTGGACAAAATGTCTGGGACT
Seq A exon
GTTCATTAAGTGTCAGCAGCAATATGGTGAGGTCCCAGCCCCAGCTTTCTGCATTTGACT
Seq C2 exon
GTTCACCAAATCCCGCAGGGTGACAGACAAATGAGGCCCCCCAAACCCAAGAGGAGGAAGATCTCCAGATAACAGAGAGTGTTCCACTGACACAGTGTTCATCAAAGGAGCATGCACAGACACACCTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000027596_MULTIEX2-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
PF0085512=PWWP=PD(22.0=69.2)
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSRNOT00000034632fB8460
Other Inclusion Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGAACTGGACAAAATGTCTGG
R:
TGTCTGTGCATGCTCCTTTGA
Band lengths:
148-208
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]