RnoEX0056975 @ rn6
Exon Skipping
Gene
ENSRNOG00000002886 | Myh10
Description
myosin heavy chain 10 [Source:RGD Symbol;Acc:71000]
Coordinates
chr10:55332055-55338553:+
Coord C1 exon
chr10:55332055-55332157
Coord A exon
chr10:55332711-55332740
Coord C2 exon
chr10:55338461-55338553
Length
30 bp
Sequences
Splice sites
3' ss Seq
TATCTGTTTGTAATGAGCAGCAG
3' ss Score
2.12
5' ss Seq
CAGGCAAGT
5' ss Score
3.1
Exon sequences
Seq C1 exon
GGGTGAATCAGGTGCCGGGAAGACAGAAAATACAAAGAAAGTCATTCAGTACCTTGCCCACGTTGCTTCTTCACACAAAGGAAGAAAGGACCACAATATTCCT
Seq A exon
CAGGAATCGCCTAAGCCAGTGAAACCCCAG
Seq C2 exon
GGGGAACTTGAACGGCAGCTTTTACAAGCAAATCCAATTCTGGAATCATTTGGAAATGCGAAGACTGTGAAAAATGATAACTCATCTCGCTTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000002886_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.229 A=1.000 C2=0.089
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.0=100)
A:
PF0006316=Myosin_head=FE(1.3=100)
C2:
PF0006316=Myosin_head=FE(4.4=100)
Main Inclusion Isoform:
ENSRNOT00000065895fB9189
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGCCGGGAAGACAGAAAAT
R:
AAAGCTGCCGTTCAAGTTCCC
Band lengths:
115-145
Functional annotations
There are 1 annotated functions for this event
PMID: 16481398
The B1-inserted isoform of NMHC II-B, which contains an insert of 10 amino acids (MmuEX0030346) near the ATP-binding region (loop 1) of the myosin heavy chain, is involved in normal migration of facial neurons. Deletion of the B1 alternative exon, together with reduced expression of myosin II-B, results in abnormal migration and consequent protrusion of facial neurons into the fourth ventricle. This protrusion is associated with the development of hydrocephalus. Restoring the amount of myosin II-B expression to wild-type levels prevents these defects, showing the importance of total myosin activity in facial neuron migration.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]