RnoEX0074049 @ rn6
Exon Skipping
Gene
ENSRNOG00000001516 | Rapgef4
Description
Rap guanine nucleotide exchange factor 4 [Source:RGD Symbol;Acc:621886]
Coordinates
chr3:58907968-58909349:+
Coord C1 exon
chr3:58907968-58908018
Coord A exon
chr3:58908794-58908870
Coord C2 exon
chr3:58909285-58909349
Length
77 bp
Sequences
Splice sites
3' ss Seq
TCTCCCAAGGATCCTTCCAGAAA
3' ss Score
-0.21
5' ss Seq
AAGGTAATC
5' ss Score
7.46
Exon sequences
Seq C1 exon
AAACTGCCGAGCAAGTTTAAGAAGTTCTATGCGGAGTTTGAGAGCTTAATG
Seq A exon
AAATCACAGGGCGTACAGGCTGACAGCAGCTAAACTGGAGCCCCCCCTCATCCCTTTCATGCCCTTGCTTATTAAAG
Seq C2 exon
ATATGACATTTACTCATGAGGGGAACAAGACATTCATTGACAATCTAGTAAACTTTGAAAAAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000001516-'60-48,'60-47,61-48
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(8.6=100)
A:
PF0061714=RasGEF=FE(15.7=100)
C2:
PF0061714=RasGEF=FE(11.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Mouse
(mm10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACTGCCGAGCAAGTTTAAGA
R:
GATTGTCAATGAATGTCTTGTTCCCC
Band lengths:
95-172
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]