RnoINT0015236 @ rn6
Intron Retention
Gene
ENSRNOG00000000437 | Agpat1
Description
1-acylglycerol-3-phosphate O-acyltransferase 1 [Source:RGD Symbol;Acc:1303287]
Coordinates
chr20:4376261-4376636:+
Coord C1 exon
chr20:4376261-4376436
Coord A exon
chr20:4376437-4376540
Coord C2 exon
chr20:4376541-4376636
Length
104 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGT
5' ss Score
7.77
3' ss Seq
TGTGCTCTGGCCTCCCTCAGGTG
3' ss Score
10.21
Exon sequences
Seq C1 exon
GAATGATGGAGGTACTGCCAGATCGCTGTGTGCCCATTGCCAAGCGTGAACTATTATGGGCTGGCTCTGCCGGGCTGGCCTGCTGGCTGGCAGGAGTCATCTTCATTGACCGGAAACGCACAGGAGACGCTATCAGTGTCATGTCCGAGGTGGCCCAGACCCTGCTCACCCAGGAT
Seq A exon
GTGAGTCTTCCTCAGATGGGGATTTGGGGATTGGGGAGCGAATGGTTGCAAACCACAATAGTCGGTGCTGTGCCCTTCCTTATATGTGCTCTGGCCTCCCTCAG
Seq C2 exon
GTGAGAGTCTGGGTTTTTCCTGAAGGAACAAGAAACCACAATGGCTCCATGCTGCCCTTCAAACGAGGCGCCTTCCACCTTGCAGTACAGGCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000437:ENSRNOT00000000500:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0155316=Acyltransferase=PD(25.0=83.3)
A:
NA
C2:
PF0155316=Acyltransferase=FE(24.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTACTGCCAGATCGCTGTGTG
R:
CTGTACTGCAAGGTGGAAGGC
Band lengths:
255-359
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]