MmuINT0012725 @ mm9
Intron Retention
Gene
ENSMUSG00000034254 | Agpat1
Description
1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha) [Source:MGI Symbol;Acc:MGI:1932075]
Coordinates
chr17:34748219-34748645:+
Coord C1 exon
chr17:34748219-34748394
Coord A exon
chr17:34748395-34748549
Coord C2 exon
chr17:34748550-34748645
Length
155 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGT
5' ss Score
7.77
3' ss Seq
TGTTTATGGCCTCCCATCAGGTG
3' ss Score
8.74
Exon sequences
Seq C1 exon
GAATGATGGAGGTCCTGCCAGATCGTTGTGTGCCCATTGCCAAGCGTGAGCTACTATGGGCTGGCTCTGCTGGGCTGGCCTGCTGGCTGGCAGGAATCATCTTCATTGACCGGAAACGCACAGGGGACGCCATCAGTGTCATGTCCGAGGTGGCTCAGACCTTGCTCACCCAGGAT
Seq A exon
GTGAGTCTTTCTCAGATGGGGACCTGGGGACCAGGGCATGAGTGGCTGCAAACCACAATAGTCAGTGCTGTGTCCTCTCAGTGCTGAGTTCTCTCAGTGCTGAGTCCTCTCAGCATGGCATGACCCCCCCTTGTGTGTTTATGGCCTCCCATCAG
Seq C2 exon
GTGAGAGTCTGGGTTTTTCCTGAAGGAACAAGAAACCACAATGGCTCCATGCTGCCCTTCAAACGAGGCGCCTTCCACCTTGCAGTACAGGCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000034254-Agpat1:NM_001163379:4
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0155316=Acyltransferase=PD(57.1=95.2)
A:
NA
C2:
PF0155316=Acyltransferase=FE(24.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGATCGTTGTGTGCCCATT
R:
CTGTACTGCAAGGTGGAAGGC
Band lengths:
249-404
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: