RnoINT0028246 @ rn6
Intron Retention
Gene
ENSRNOG00000054274 | Cacnb3
Description
calcium voltage-gated channel auxiliary subunit beta 3 [Source:RGD Symbol;Acc:2248]
Coordinates
chr7:140319745-140320227:+
Coord C1 exon
chr7:140319745-140319867
Coord A exon
chr7:140319868-140320104
Coord C2 exon
chr7:140320105-140320227
Length
237 bp
Sequences
Splice sites
5' ss Seq
AAGGTGTGT
5' ss Score
6.64
3' ss Seq
CCCATGATCAAATTCCCCAGCAC
3' ss Score
3.76
Exon sequences
Seq C1 exon
GGTTCAGCCGACTCCTACACCAGCCGCCCCTCTCTGGACTCAGACGTTTCCCTGGAGGAGGACCGGGAGAGTGCCCGGCGAGAAGTGGAGAGTCAGGCTCAGCAGCAGCTGGAAAGAGCCAAG
Seq A exon
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGGTGGGAGGCTAGGCTGGGCACGAATCTGTGCAGAATCTTAGGTCCCCAGGGGCATGTCAGGGTGTAAGTCACCTGTGGAAAAGTGTATGCCTGTAGCAGCTTTCTTGTCTGGGCCTCTGCCTCCCCCTGAGGTACTGTGAGGTGACTCTCTCTCACGGCCCCATGATCAAATTCCCCAG
Seq C2 exon
CACAAACCTGTGGCATTTGCTGTGAGGACCAATGTCAGCTACTGTGGAGTTCTGGATGAGGAATGCCCAGTCCAGGGCTCTGGAGTCAACTTCGAGGCCAAAGATTTTCTGCACATTAAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000054274:ENSRNOT00000081206:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.976 A=NA C2=0.024
Domain overlap (PFAM):
C1:
PF120523=VGCC_beta4Aa_N=PU(93.0=97.6)
A:
NA
C2:
PF120523=VGCC_beta4Aa_N=PD(2.3=2.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTTCAGCCGACTCCTACACC
R:
CTTTAATGTGCAGAAAATCTTTGGCC
Band lengths:
244-481
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]