RnoINT0034864 @ rn6
Intron Retention
Gene
ENSRNOG00000021138 | Cers2
Description
ceramide synthase 2 [Source:RGD Symbol;Acc:1310059]
Coordinates
chr2:196493542-196493739:+
Coord C1 exon
chr2:196493542-196493599
Coord A exon
chr2:196493600-196493688
Coord C2 exon
chr2:196493689-196493739
Length
89 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGT
5' ss Score
7.77
3' ss Seq
TTTAATCTCTCTCTTTGCAGAAA
3' ss Score
12.38
Exon sequences
Seq C1 exon
CTGGAGATTCACATATTACCTCATTGCCTTTGTTGCTGGCATGGCTGTCACTGTGGAT
Seq A exon
GTGAGTACTGGGAAGTGGGAGCACATTCTTTAAGATGGGGTAAAGCCATAGGATGTGAGTCCTTCTTCCTTTAATCTCTCTCTTTGCAG
Seq C2 exon
AAACCCTGGTTCTATGACTTGAGGAAAGTTTGGGAAGGATATCCCATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000021138:ENSRNOT00000028699:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0379811=TRAM_LAG1_CLN8=FE(9.8=100)
A:
NA
C2:
PF0379811=TRAM_LAG1_CLN8=FE(8.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGATTCACATATTACCTCATTGCC
R:
TCCTTCCCAAACTTTCCTCAAGTC
Band lengths:
95-184
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]