RnoINT0049001 @ rn6
Intron Retention
Gene
ENSRNOG00000048363 | Dnah5
Description
dynein, axonemal, heavy chain 5 [Source:RGD Symbol;Acc:1560828]
Coordinates
chr2:80977299-80978870:+
Coord C1 exon
chr2:80977299-80977475
Coord A exon
chr2:80977476-80978756
Coord C2 exon
chr2:80978757-80978870
Length
1281 bp
Sequences
Splice sites
5' ss Seq
AAGGTTCGT
5' ss Score
8.44
3' ss Seq
TTCTTCTGTGTTGTGCTTAGGTT
3' ss Score
11.02
Exon sequences
Seq C1 exon
ATCCTCGCTGAGAACAATCAGCTGCGGAAGGAAGCAGATGATGTTGGGCCCCGAGCAGAGTTGGAGCACTGGAAAAAAAGGCTCTCCAAATTTAATTACCTTTTAGATCAATTGAAAAGCCCCGATGTGAAGGCCGTGCTGGCGATGCTTGCCGCGGCAAAGTCCAAGCTTCTGAAG
Seq A exon
GTTCGTAGCCATTTTGCCACGATTTGAAGTATGTGAATAGGAGAGTTCTCCGTCCCCAACTCCACAGAGCAATGAGGAACAGAAAGGTGTTATTTTACCAGTGTGAAAATCTTTAGGACTGGGTAATAGCTGTCACCTAACTTTGGGTACAGCCTTTTGGGAATCAGCCTTCGAGTTAAAGACATTTGTTGGCTGTTGCACGCACATGCACTATTAGTGAGCGTTGCAATTAGTGAGATACAATGCGATCACACGACTAGACAAGAACACATGCACACATTCAGACAATACAGATGCAGCAACTGTGAAGTCTTAAGTCCTCATGTATTACTTGTATACTGCAAAGCACATAGCCCCAAAGCTCATGAGCTGAAAACAGAATTTTTACCTCAGACCAGCAATTGCCGTGTAGCTGAGCCAGCATCTTCTGACTCAAGGTTCCTAGGAGGCTGCATCTAAGCTGTTGGCTCCTTTGCAGGTTCACACAGGGAAGGCTTGCTTCTAAGTTTGCTGCTGACAGGGCTGCTCTTTGTTGGTCAGAGGCATCCTTCAGTTCTCATCACATGCTCCCTTCCATGGGCAGCTCATGGGTGGGGCACCAGGCTTTCTTTAAAGAGAAGTACAGAACGTGTGAGAGGGCACCATGTCTAGAAGGCACATTGAAAACTAACACCCGGGAGCAAAGCATCATTACTTCTGTACTACTCTATTCATTAGGAGCAAGTCACTGTTTTAGAGAGACTCTATACATCGTGCTACAGTATAGTCCAGAATTACTACGATTAGGATTTGCATAAAGCCAATTCTGAATGCTACATCCCCTTTACTATGTAGATAGCTTTCTATTTTAATGTCCCAACAGATAGTTGGTAGTTAAAATAAATACTTGGTGTGTCATCAAATGGACACAGGTATAGGGCCACAAGGATGGGGGTTGTGGGAGGGAGAACTCAGGTTGGAATGGAGAGGATGTGGAGAGCAGCAGGAGACAGGAAGCTAGTCTGGTGGCCACACTGCAGGCTGCTGTCATTTTTTTTTTTAACAGGGCTATGCTAGTCTCTGCTAGAATAGAATATACAAGAGAAATCACAAAATAAATGTCCTTAAATATTTAATTTTTTTGACTTGCTCTTCCACATTCTTCTAAGATTCATTATTTTAATTGCAGGAGGCAGGAAGCAAGCAGAGATGCTGATGGGAATTGGAGTGATGCTTAGTATCTCTTATTTTGCAATCATCTGGGATAAGTGTGTGAACCTTGTTCTTCTGTGTTGTGCTTAG
Seq C2 exon
GTTTGGCGGGATGCAGACATTAGAGTCACTGATGCAGCCAATGAAGCAAAGGACAATGTCAAATATTTGTACACACTGGAGAAATGCTGTGACCCTCTGTACAGCAGTGACCCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000048363:ENSRNOT00000074689:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.017 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF083857=DHC_N1=FE(10.4=100)
A:
NA
C2:
PF083857=DHC_N1=FE(6.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAATCAGCTGCGGAAGGAAG
R:
TGCTGTACAGAGGGTCACAGC
Band lengths:
270-1551
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]