RnoINT0049191 @ rn6
Intron Retention
Gene
ENSRNOG00000000542 | Dnah8
Description
dynein, axonemal, heavy chain 8 [Source:RGD Symbol;Acc:619986]
Coordinates
chr20:9421486-9423228:+
Coord C1 exon
chr20:9421486-9421673
Coord A exon
chr20:9421674-9423141
Coord C2 exon
chr20:9423142-9423228
Length
1468 bp
Sequences
Splice sites
5' ss Seq
ATGGTGAGA
5' ss Score
7.23
3' ss Seq
GAATATATCTCAACTCCTAGAAC
3' ss Score
5.06
Exon sequences
Seq C1 exon
GCCTTGCCCAGTCCGGTTCCTGGGGTTGCTTTGATGAGTTCAACAGAATTGAATTGCCTGTTTTATCTGTGGCGGCCCAACAAATTTACATAGTTCTGACAGCGAGGAAAGAGAGGAAAAAACAGTTCATCTTCTCAGACGGCGATTGCGTGGACTTAAATCCAGAATTCGGAATCTTCCTAACCATG
Seq A exon
GTGAGAACACGGGTTTTAAGCGCAAGTTGGAGAGTTTAATTGTTGTAGATATAAATAGACAAGTCCCTGTATCACAGGATAAACGAGCCAGAAGTCATGGATTCTCTCTTTCTTGTGGGTATTTAAAAGATTTTTAGATTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACGCATGTGAGTGTAGGTGCCTGTTGAGGCCAGAAGGGGGCGTTGAATTCCTCGGAGCTGAGGTTTATAGGTTGCCTGCTGCTATAGCAAAGAAAACCATCATGCCTGGCTTGGAGTTTCTGTCTAGAAAGGACATATCTAGTTCTCCTGGGACAAACTACACCTACCCACAATGGGGACTGGAGGGTGAGCGCGATTATCAAAGAGCAAGGACGGAACACAGATGCCCACTATGTCATCCTAGAGCTGCGGTATCCTCTGAGTATCATCTCATAGCTGAGATACTGCAGGCTCAAATCGCACAGCCAGTAAGAGAAACTAGCTATTGTGCCGTGTGTGTGTGTGGTGTGTACGTGTGTATGCGTTTCCTGTGCATGTGTGTCTATGCATTTTGTGTCTGTGTGTATGCATTGTGTGTAATGCATGCAGTGGGGTGCCCGCGCGTGTGCACACACACACACACACACACACACACTTGCATGTGCACCCATGGTATCGGAGTGGGCCATAAGGTCAATATCGAGTCTTTCTGTGTTGATAATCTTATGGTTTTTTTTTTTTTCCTTTCTTTTTTTCAGAGCTGGGGACCGAACCCAGTGCCTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTAATCTTATGTTTTGAAACTTAGTCTCTTACCCATTACAGATGGTTGTAAGCCACCATGTGGGTGCTGGGAACCAAACCCGGGTTTTCTGCAAGAGCAGCCAGTCAGTGCTCCGACTCACTCAACCGTCTCTCCAGGCCCTCCCTTTTAATAACATATTTTAGATTGTAGTTGCTTGGGTCTATTTATCAGCCCTGCTCCATGTTTTAAACCAGCAGACACACAGGTGCTTACACTTTTTTTTTTTTTTTTGCTGTGTAAAACTATGTGCAACTTTGATCATGAATTGAAAATGAGGTGTATTTTAAAAAGGTATGATGTGAAATTACATCAAATACATAATAAAAACATACTAAATCAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAAGAAGGTCTGTCAAGGCCCTGGGTTCGGTCCCCAGCTCCGAAAAAAAAGAAAGAAAGAAAAAAAAAAACGTACTAGATCAAATGGACCAGTATACATTTTTGAGACCCCAAGGGCATTTTTACAAACCTAATGTTTCTGTGTATGGCCTCGGAATATGAATTGGGGGGGCTGAAATCATTTATGTCTGGGAAATAAAAACGGAACAAATAATCGAATATATCTCAACTCCTAG
Seq C2 exon
AACCCCGGATACGCTGGGCGCCAGGAACTGCCCGAAAACTTAAAAATCCAGTTTAGAACAGTTGCGATGATGGTTCCTGATAGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000542:ENSRNOT00000000651:56
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF127742=AAA_6=FE(26.7=100)
A:
NA
C2:
PF127742=AAA_6=FE(12.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTGCCCAGTCCGGTTCCT
R:
GCCTATCAGGAACCATCATCGC
Band lengths:
271-1739
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]