RnoINT0064444 @ rn6
Intron Retention
Gene
ENSRNOG00000061527 | Gck
Description
glucokinase [Source:RGD Symbol;Acc:2670]
Coordinates
chr14:86155063-86155365:-
Coord C1 exon
chr14:86155270-86155365
Coord A exon
chr14:86155163-86155269
Coord C2 exon
chr14:86155063-86155162
Length
107 bp
Sequences
Splice sites
5' ss Seq
GGGGTGAGC
5' ss Score
5.97
3' ss Seq
GTCTCTGTACTTCTTGGCAGGAC
3' ss Score
8.18
Exon sequences
Seq C1 exon
GGCATCCTCCTCAATTGGACCAAGGGCTTCAAGGCCTCTGGAGCAGAAGGGAACAACATCGTAGGACTTCTCCGAGATGCTATCAAGAGGAGAGGG
Seq A exon
GTGAGCACAGCTTTGCCTGCCCTGAGGGGCTGTCCTGAGCATGTCTGCCTTCATCACAGGGCCCCTCTCCTCAGCTCCATGTCCATAGTCTCTGTACTTCTTGGCAG
Seq C2 exon
GACTTTGAGATGGATGTGGTGGCAATGGTGAACGACACAGTGGCCACAATGATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000061527:ENSRNOT00000080147:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(20.1=100)
A:
NA
C2:
PF0034916=Hexokinase_1=PD(14.9=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCATCCTCCTCAATTGGACC
R:
CACAATCATGCCGACCTCACA
Band lengths:
195-302
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]