RnoINT0099937 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37698835-37700401:-
Coord C1 exon
chr3:37700297-37700401
Coord A exon
chr3:37698940-37700296
Coord C2 exon
chr3:37698835-37698939
Length
1357 bp
Sequences
Splice sites
5' ss Seq
GAGGTACGT
5' ss Score
10.2
3' ss Seq
TTAATATGATGTTTGAGCAGAAA
3' ss Score
3.6
Exon sequences
Seq C1 exon
TATAAGTATAAAGAAGTATTTGAAAGAACAAAGTCAAACTTCAAGTATGTTGCTGACTGTCCAATTAACAGGCATTTCAAGTACGCAACTCAGTTGATGAACGAG
Seq A exon
GTACGTAAAACTAATCTGATTAAACCTATTTGGTTCTCTTATATTTTTGGTTGTGAGCCTAGCCTTTAACGGTTGAGCCATCTCTCCAGCCCCCTATTTGGTTCTCTTAAAAAATTTGCAGGGCTGTTTCATTAAAACCTGATATATACCATGACAGGAAATCTGTCCTAAATGTTCCTTCCATATAAGGGCTTGGAAAGACTGTACAATGTCACTATCTGGTTGGCCCTGCCCAAACTTTGAGTAGATCCACGTTCACCTTCACCTCTGTAACACAGTCTTAAGGTGCCAGAGAGAGGAGGAAGGATTTTGTGGACGATCCACTAACTGCCTTTAACCCATGTAGAGCTTGGAGACCTGGATGGTCAAGGTTGATTCAGTAGCCCCACAAAGGCACCAAAATGGAACTGGTCAGAACCCTGACGCGCTCAATGGGGATAGTTGTGTGACTCATGCATTCAGACACATCACTGTTACTCCCACGGAATTGGATGCATACGGGTATCTATTTACACCTCCCTCTATATTCTACTCCGTCTTCCCTATACCGATTTTACAATGATGTAATTTGAATATAATTCGAGCGAGCCCAAATCATCAAGCTGTGAGCCCTAAATTGGGGAGTTTTTATAATGTTTAGATGTTTTCATAACTAAACTTACGCTTTTCTGCTTTCAGATAATACGTGGACTTGCATAAGTTCAAACATGTTAGAATATTACTAGAGGGGCACTAACAATAAGACTGTAGCCAGCGGTGCATTTCCGTGTGGCCTTTGCCAATTTCAGTTCATTTTTGTTTAATTACTTCATTTCAACACATTTCCAGGCAGGAAAAATCTAACACCAACACCATCAGCAGCTTCCTAGGACAGTCTTGATATATTTTGTTTTTCCTGTTTCATGAATAATTTCCAGGTATCTTTTAAATCATCAAGTAAATCAGTACAGTTTTCCTTGAGTAAACCTAGTTACAATTTTGGATATTATAGTTAGTTACACCTCTGTATAGCATTAATTATACTTTTAGACTCTTGCTGCCTTGTAGATGAAACTTGTAGATTATTGGCACCTCATTTTAAATACAGTGTAAGATTCCGTCCAGCCTGATGTCACTAACATGTATAATGAAAAGATAGAAACGTGACCCTACCATACCTCAGCTTGCTCTCTTCCAGGCCTCAGCCTCTCTCCGGAAAAAATCTGTGTTCGCTTGTCATTCCTACTCTCTATATGATCATCTAAACATTGTCCTGGGTATTGCTAGGTTTCAGACTCTCTGGAGTATTCAGATTGAGACATTACCATGTGCCCTCAATGTCATTTTCTTGCTATAATTTAATATGATGTTTGAGCAG
Seq C2 exon
AAAAAATACAGAGCTGATTATGAGCAGCGGAAAGATAAATACCACCTGGTAGTCGATGAACCTAGACATCTGCTGGCTAAGACGGCAGGCGACCAGATCAGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:79
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAAAGAACAAAGTCAAACTTCAAGT
R:
CTGGCTGATCTGGTCGCCT
Band lengths:
190-1547
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]