RnoINT0099953 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37685393-37686939:-
Coord C1 exon
chr3:37686835-37686939
Coord A exon
chr3:37685498-37686834
Coord C2 exon
chr3:37685393-37685497
Length
1337 bp
Sequences
Splice sites
5' ss Seq
ATCGTAAGT
5' ss Score
10.44
3' ss Seq
ATAGACCTTCTCATCACTAGGTG
3' ss Score
6.35
Exon sequences
Seq C1 exon
TACCAGTACAAGAAGAACTATGAGAAGAGCAAAGGCCACTACCACACGATACCCGATAACCTGGAACAGCTACACCTCAAAGAAGCCACAGAGCTGCAGAGTATC
Seq A exon
GTAAGTCTATCAAGATGGCCTCCCCACACTGAGCTTCCATTTATCACAGTATTAAAAAACAGTATCAAGTCCACTCTAGGAACGGGTGGACTCATTTTAATAGCCATTGTTTGAGAAAATATTCCCCGGTACTGGGGCTTGAATTGGGTAACATTTTGAGATTGGTTTATTGACAGTATTTATATATAATAGAGAAATATACAGCTGCCATGTTTGTTATTCATGACAGTGCTGGAAGCCTGGCAGTTAGTTCCCCAGAGAGACAAACCTCTCACAGCAACCCTATGTCCTCAGCCAGGAAACACCAATAAATTGGACAAGTGACATGATCACACCCAACACTGTAGGAGTCTCTGAGTGTTATAATTATTCTTAGAGGAAAGAACTTACTAGTAACAAAGTCTTGGTACCACAAACTCATCATTATTGGGTGTCCTGGGGTATTAGCCAGAGGGCTGAGTTATGATAGGTGCTTCCCCATCATGTGTAAAGCAGTGTGTGTCCTCTTCCTGTGTTCCATCATGACCAAGAACGCTGAGTTGAGCAACGGCATTGAGGTTATGGGTGGAAGAGCCCTTGTCTTTCTACCTCTCAGTTTCTCCTCATGCCATTATCAATAGTGTGTTGACTATAAGGAAGAGACCATTTTACCTGCTAATGTCAGGCTTACTCAGTCCAGCTGTGAATTGCTTTCCCCTTAAATGCTAAGTTATCACAAATAGTAAAGTACATAGACACTGGTGTCCCAGAGGGAGCTGTTTGCTTTTAAAGCCTCACATGTTCTACAGTCAATGGAGACCACAGGTCTTTGGTAATAATAGTTCTTATGAAAGAACTAGAGGCTTTATCAGAAATATCAAAAGATGCTATTTATTGTCAAAATTCATATATCCATATCAAGTCTTGGATGGCCTCATATACGGGCAATTTTCTCCAAACAGCATTTCCAAAAATCAATATTTTTGCCAGATAATTGTTGCCAGTGATTCTTTTGTGTCTCTAAATTAAGTATATATTAAATCTAATTAGCTCGTTCTTTTCAGATAGCATTGATTCTCCATCCACAGGCATCAGCTCAAAACGGTCTACGCCTGTAACACAATAGGAAAGTCTTTTGGCTTTGCAATAAGATAAGCCACAATAGACACAATCGAAAGTATGTAGGAAGCTGAGGCTAGAGGATTCTGAGTTCAAAGCCAGCCTGTGACACATTGTGAGAACCTATCTCAGATAATATTAAAAAAATTAAGTAAACCAATAAATTGCTGCCTGTAAATAGTGTCTCTGTCTAGGAATTGACTAGTGGCAAAATACTCAATAGACCTTCTCATCACTAG
Seq C2 exon
GTGAAATACAAAGAAAAATATGAAAAAGAACGAGGCAAGCCCATGCTGGACTTTGAGACTCCAACATACATCACTGCCAAAGAGTCTCAGCAGATGCAGAGTGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:93
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.486 A=NA C2=0.686
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCAGTACAAGAAGAACTATGAGAA
R:
CCCACTCTGCATCTGCTGA
Band lengths:
209-1546
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]