RnoINT0100355 @ rn6
Intron Retention
Gene
ENSRNOG00000009377 | Nelfb
Description
negative elongation factor complex member B [Source:RGD Symbol;Acc:1307832]
Coordinates
chr3:2423174-2423527:-
Coord C1 exon
chr3:2423385-2423527
Coord A exon
chr3:2423281-2423384
Coord C2 exon
chr3:2423174-2423280
Length
104 bp
Sequences
Splice sites
5' ss Seq
CAAGTAGGA
5' ss Score
4.46
3' ss Seq
CCATGGCTTCTCTCATGCAGGTT
3' ss Score
9.04
Exon sequences
Seq C1 exon
GAGGCAGAGCTCATCACCAAGTTCCTGCCGATGCTCATGTCCTTCGTGGTGGATGACTACACTTTCAACGTAGATCAGAAGCTTCCAGCTGAGGAGAAAGCCCCAGTCACATATCCCAACACGCTTCCTGAAAGCTTCACCAA
Seq A exon
GTAGGACTCCAGGGCAGGGTTTGGGGGAGGCACTCGAGTGGAGCTGACAGGGAAGTTGCTGCTGGCAGGCTGACTCTTGGAAGACCATGGCTTCTCTCATGCAG
Seq C2 exon
GTTTCTGCAGGAGCAGCGCATGGCTTGTGAGGTGGGACTGTACTATGTTCTACACATCACCAAGCAGAGAAACAAGAATGCTCTCCTGCGCCTGCTGCCTGGTCTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000009377:ENSRNOT00000012978:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.021 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF062098=COBRA1=FE(9.9=100)
A:
NA
C2:
PF062098=COBRA1=FE(7.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGGCAGAGCTCATCACCAAG
R:
CAAGACCAGGCAGCAGGC
Band lengths:
250-354
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]