RnoINT0100565 @ rn6
Intron Retention
Gene
ENSRNOG00000012512 | Nexn
Description
nexilin (F actin binding protein) [Source:RGD Symbol;Acc:708354]
Coordinates
chr2:257453646-257455751:-
Coord C1 exon
chr2:257455566-257455751
Coord A exon
chr2:257454004-257455565
Coord C2 exon
chr2:257453646-257454003
Length
1562 bp
Sequences
Splice sites
5' ss Seq
AAAGTACGT
5' ss Score
7.99
3' ss Seq
ATACACTTATTTTAATACAGAAA
3' ss Score
4.09
Exon sequences
Seq C1 exon
GATGATGATGTTGATGTAAAACCTGCAAAGAAGAGTGAGTCTCCCTTTACTCACAAAGTGAACATGAAAGCCAGATTTGAACAAATGGCTAAGGCAAGACAAGAAGAAGAGCAAAGGAGAATTGAAGAACAGAAGTTACTGCGGATGCAGTTTGAACAGAAAGAAATCGATGCAGCACTTCAGAAA
Seq A exon
GTACGTATAAATGGATTTGGTGTTCTAGACACATCTGTGAGCAATTAAGAAATGTGATTTCTACCAAGTGAGATTATACAATCCATTTTAGCTATTTCTTCTCTGTTCAATTCTGACCATTTCTAAATTCCCTAGAAAGGCAGCCTAGAATAACCACCTGCAGACCCCCAACCCAGGGAATGGGGACTATAACTTCTTCCTGGTGAATATGGACATTGAGGTATCTTTGAAGGGGGTGGGTAGGGAAAGTGGGGGGGTTGGTTAGGCTTTAAGAAGGCCATGCCAATGATTGTTGATAGCCTCTGATGTCTGTTTTGACTGATTCGTCTGAAAACCCATGACAAGCAGGTCAGTTTAGCGTGTCTGAAGACAAAATCTCACCAAAGCTTCATATTCTACAATATACAAATCTCAAAAACAGAATTTAAATACCATCAAAGTATCTGTATGGATTGTGTTAAGTCTGTGTAGGGTGTACAGACTAGTCAAGATGAAAACTGTTTGAACATGTCTTTTTTATGAGTCAACTTTAGTATTTGTGCCACATGAAGGGTCTCCCTGTCATATCTGATCCATATATCTGATCGTATCTGGAAGGTGTCCAGAGTCTATATCCCCTTTTCTAAAAACACAAAGCCTTTCTCCCAAATTAGTATGTTCTTTAGCCAGTAACAAGGAAAACTTTTAGCTTGATCTCTCTCCCAGATGAATAAAGTAATTACAAAACCAACCACACCAATCTTGATAGCAATCATAACAAGTAAACACAGTAGAGCATATGACGCAGGCTTTTGTAATTTGTGAAACCAGGTTTTTTAATCCAAAATTCTTGTGGATCCTCTAAGACTTGATCTTCAGTCCTTCCATCTTTCCTCCTTGGAGGCTGTTGCTTTTTCTGCTAGTTAGCCTGTCCTTGTACTGCAGGGGTTCCCAGCACACTTGGGAACTTTCAACATAATTAAACCAATCTTTTTATTAATACCTGCTTATAGGAAGCAGTTAACTTTTATAGTTTCTATTTACAGCAGGGGGGATTCAGTGGCTGAACTGGGACTACCCGTGTTAGACTCCTTGACCCCTCTCCACTGGCTAAGAACTAAGTCTCCCACCCTTAGGCTAAGAATTCCTAATGGAATTTTGCCTGCCACTCAGATGTCATCTGTGACATGGAGTTTATAAACCTATGCTATGGCTAGCCAGGCACTGGCAAAGGCAGTCTCCTTATCACCAGAGTAAGCCCCAAGTGGGAGTCTGCCCTGACTTCATCTGAGTATGGTCACCCCTTGAGAGAAACAAAAAACAACCAACCAACCAACCAACAAAAAACCCCCAAAAACAACCAAAAAAAAATAAATAAAATAAAACAGACAGCACCCCACAACAGCTTTCAAGCCACTGGAATGGTTTAGGTAGTAAACCAACTCCTTTCTATGTCAATGAAACCTGGGGCATCATGCATGCCAGCCAACACTCGTTATATCCCAAGCTTCCTTTTTATTTAACCTTTAAAATTATATTACTAAAATAAATGCCATCTTGATAATACACTTATTTTAATACAG
Seq C2 exon
AAAAGAGAAGATGATGAGGAAGAAGAAGGTAGCATTGTTAACGGCTCCACTACTGAAGACGAAGAGCAAACCAGATCAGGAGCCCCATGGTTCAAAAAGCCACTAAGAAATACCTCAGTTGTAGACAGTGAGCCAGTGAGATTCACTGTGAAAGTGACAGGAGAACCCAAGCCAGAAGTTACGTGGTGGTTTGAAGGAGAACTACTGCAGGATGGAGAAGACTATCAGTACATCGAAAGAGGAGAAACTTACTGCCTGTATTTACCAGAAACTTTCCCCGAAGATGGAGGAGAATATATGTGTAAAGCAGTCAACAGTAAAGGCTCAGCAGCGAGTACCTGTATTCTTACCATTGAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000012512:ENSRNOT00000016967:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.803 A=NA C2=0.375
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0767911=I-set=WD(100=75.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACAGAAGTTACTGCGGATGCA
R:
TCCTCCATCTTCGGGGAAAGT
Band lengths:
350-1912
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]