RnoINT0112270 @ rn6
Intron Retention
Gene
ENSRNOG00000011317 | Pkn2
Description
protein kinase N2 [Source:RGD Symbol;Acc:620146]
Coordinates
chr2:248738274-248738667:-
Coord C1 exon
chr2:248738560-248738667
Coord A exon
chr2:248738355-248738559
Coord C2 exon
chr2:248738274-248738354
Length
205 bp
Sequences
Splice sites
5' ss Seq
AGGGTAAGA
5' ss Score
9.21
3' ss Seq
CTTTCATCTTAACTTCCTAGCTG
3' ss Score
7.45
Exon sequences
Seq C1 exon
TCTCCCTTTCCTGGTGATGATGAAGAAGAGGTTTTTGACAGTATTGTAAATGACGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCCATAATGAGAAGG
Seq A exon
GTAAGAAATTAGGAACAAGAATTTTTTTTTTTAATTTAAACTTAAGCTTTTTGTTGAAAATTTCCCAGTTGAATTTTAGAAGCTATTTTTAATGTGTAGATGTTGCATGCTTTAGACAGATCTAAACACAAGGTCCTACATCTTTGACAGTAGTGAGACCACGGTTGGTGGCACATGTTTTATTGCTTTCATCTTAACTTCCTAG
Seq C2 exon
CTGTTGAGGAGGAATCCTGAGCGGCGCCTGGGAGCTGGTGAGAAAGATGCCGAGGATGTAAAGAAGCACCCGTTTTTCCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000011317:ENSRNOT00000031748:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.028 A=NA C2=0.185
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(13.5=100)
A:
NA
C2:
PF0006920=Pkinase=PD(9.6=92.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCCCTTTCCTGGTGATGATGA
R:
CGGAAAAACGGGTGCTTCTTT
Band lengths:
188-393
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]