RnoINT0142834 @ rn6
Intron Retention
Gene
ENSRNOG00000025711 | Spout1
Description
SPOUT domain containing methyltransferase 1 [Source:RGD Symbol;Acc:1563514]
Coordinates
chr3:8747385-8747706:-
Coord C1 exon
chr3:8747657-8747706
Coord A exon
chr3:8747516-8747656
Coord C2 exon
chr3:8747385-8747515
Length
141 bp
Sequences
Splice sites
5' ss Seq
CAGGTACGG
5' ss Score
10.88
3' ss Seq
CCCTCCTGTCCCCTGCCCAGGAA
3' ss Score
9.17
Exon sequences
Seq C1 exon
GTATCTGAGGAAAGCGTTCTTCCCCAAGCACCAGGATCTGCAGTTTGCAG
Seq A exon
GTACGGCTGCTGTGCCTGTCCCATCCCCCCTGGCTCATCCTCAGGCACTGAGAAATCATCCCGCCCCACAGACACCTCCAGGTAGGAAGGCAGGCCCAGAGTAGAGCCTGGCCTGACGTCACCCTCCTGTCCCCTGCCCAG
Seq C2 exon
GAATCCTCAACCCGTTGGACAGCCCTCACCACATGCGTCAGGATGAAGAATCTGAGTTCAGAGAAGGCGTCGTGGTGGACCGGCCCACCAAGGCTGGCCACGGCTCTTTGGTCAACTGTGGAATGAAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025711:ENSRNOT00000030623:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.409
Domain overlap (PFAM):
C1:
PF0259812=Methyltrn_RNA_3=FE(5.8=100)
A:
NA
C2:
PF0259812=Methyltrn_RNA_3=FE(14.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGAGGAAAGCGTTCTTCCC
R:
CTTCTTCATTCCACAGTTGACCA
Band lengths:
178-319
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]