BtaEX0009213 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000014546 | CLEC7A
Description
C-type lectin domain family 7, member A [Source:HGNC Symbol;Acc:HGNC:14558]
Coordinates
chr5:100283216-100286031:+
Coord C1 exon
chr5:100283216-100283314
Coord A exon
chr5:100284529-100284666
Coord C2 exon
chr5:100285880-100286031
Length
138 bp
Sequences
Splice sites
3' ss Seq
ATTGTTGTTGGTATATGAAGGTA
3' ss Score
3.08
5' ss Seq
CAGGCAAGG
5' ss Score
3.33
Exon sequences
Seq C1 exon
GGCTTTGTGCTGCATCCTCTCATTGGCGTCTGATTGCTGTGACTCTGGGCATTTTATGCTCAGTGATGCTGGTGATAACTGTGGTCCTGAGTACCTCGG
Seq A exon
GTATTTGGAGATCCAGTTCAGGGAACAACCTGTTGAAGAGTGACAGCTTTCCATCAAGAAATAAAGACAACCAGAGTCAACCCACACAATCATCTTTAGAAGATAGTGTGATACCTACCAAGGCTCTCACGACCACAG
Seq C2 exon
GAGTTTTCTCTAGCTCTTGTCCCCCTAACTGGATCACACATGAGGATAGCTGTTATCTATTTAGCACACTATTAGATTCCTGGGATGGAAGTAAAAGACAATGCTTTCAACTGGGCTCCCATCTCCTGAAGATAGACAGCTCAAAAGAGTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014546_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.489 C2=0.000
Domain overlap (PFAM):
C1:
PF083915=Ly49=PU(82.4=82.4)
A:
PF083915=Ly49=FE(60.5=100)
C2:
PF083915=Ly49=PD(14.7=9.8),PF0005916=Lectin_C=PU(25.2=52.9)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTTGTGCTGCATCCTCTCA
R:
TTGAGCTGTCTATCTTCAGGAGA
Band lengths:
242-380
Functional annotations
There are 2 annotated functions for this event
PMID: 11567029
[Negative results]. The two major isoforms differ by the presence of a stalk region separating the carbohydrate recognition domain from the transmembrane region and are the only isoforms that are functional for beta-glucan binding.
PMID: 16622020
Overexpression of dectin1-1B results in significantly lower zymosan association compared to the overexpression of dectin-1A at 4?C, 25?C, and 30?C but similar zymosan association at 37?C.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development