BtaEX0009270 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000019414 | CLK4
Description
CDC-like kinase 4 [Source:HGNC Symbol;Acc:HGNC:13659]
Coordinates
chr7:41114265-41119390:-
Coord C1 exon
chr7:41119230-41119390
Coord A exon
chr7:41115576-41115634
Coord C2 exon
chr7:41114265-41114487
Length
59 bp
Sequences
Splice sites
3' ss Seq
GTGTCCCCTCTCTCCCTCAGTGC
3' ss Score
10.43
5' ss Seq
CAGGTAGAG
5' ss Score
7.1
Exon sequences
Seq C1 exon
ATGCGGCATTCCAAAAGAACTCACTGCCCTGATTGGGATAGCAGAGAAAGCTGGGGACATGAAAGCTACAGTGGAAGTCACAAACGGAAGAGGAGATCCCACAGCAGTACGCAAGAGAACAGATATTGTAAACCACATCACCAGTTTAAAGAATCTGATTG
Seq A exon
TGCTCCATGTGCATCCCTCTTGAAGCTTCACACTCTGTTGAAGAGGACTCTCATCCCAG
Seq C2 exon
TCATTATTTAGAAGCAAGGTCCTTGAATGAGAGAGATTATCGGGACCGGAGATACATTGATGAATACAGAAATGACTACTGCGAAAGATATGTTCCTAGACATTATCACAGAGACGTTGAAAGCAGTTTTCGAATCCACTGCAGTAAATCTTCAGTCCGAAGCAGGAGAAGCAGTCCTAAAAGGAAGCGTAATAGACACTGTTCAAGTCATCAGTCACATTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000019414_MULTIEX1-2/2=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.407 A=0.000 C2=0.321
Domain overlap (PFAM):
C1:
PF081797=SspP=WD(100=51.9),PF050777=DUF678=PU(72.3=63.0)
A:
PF050777=DUF678=PD(25.5=57.1)
C2:
NO
Main Inclusion Isoform:
ENSBTAT00000025863fB1771
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGGAAGAGGAGATCCCACAG
R:
TCAATGTATCTCCGGTCCCGA
Band lengths:
138-197
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.903, FDR=0.138), PC9 No Change at 14 days (FC=1.057, FDR=0.478), Late Xenograft Enriched (FC=1.934, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development