HsaEX0015709 @ hg38
Exon Skipping
Gene
ENSG00000113240 | CLK4
Description
CDC like kinase 4 [Source:HGNC Symbol;Acc:HGNC:13659]
Coordinates
chr5:178618556-178623416:-
Coord C1 exon
chr5:178623256-178623416
Coord A exon
chr5:178619838-178619896
Coord C2 exon
chr5:178618556-178618778
Length
59 bp
Sequences
Splice sites
3' ss Seq
GTGTCCCCTCTCTCCCTCAGTGC
3' ss Score
10.43
5' ss Seq
CAGGTAGAG
5' ss Score
7.1
Exon sequences
Seq C1 exon
ATGCGGCATTCCAAAAGAACTCACTGTCCTGATTGGGATAGCAGAGAAAGCTGGGGACATGAAAGCTATCGTGGAAGTCACAAGCGGAAGAGGAGATCTCATAGTAGCACACAAGAGAACAGGCATTGTAAACCACATCACCAGTTTAAAGAATCTGATTG
Seq A exon
TGCTCCATGTGCATCCCTCTTGAAGCTTCGCACTCTGTTGAAGAGGACACTCATCCCAG
Seq C2 exon
TCATTATTTAGAAGCAAGGTCCTTGAATGAGCGAGATTATCGGGACCGGAGATACGTTGACGAATACAGGAATGACTACTGTGAAGGATATGTTCCTAGACATTATCACAGAGACATTGAAAGCGGGTATCGAATCCACTGCAGTAAATCTTCAGTCCGCAGCAGGAGAAGCAGTCCTAAAAGGAAGCGCAATAGACACTGTTCAAGTCATCAGTCACGTTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000113240_MULTIEX1-2/3=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.477 A=0.000 C2=0.341
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAGTCACAAGCGGAAGAGG
R:
CAACGTATCTCCGGTCCCGAT
Band lengths:
148-207
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.903, FDR=0.138), PC9 No Change at 14 days (FC=1.057, FDR=0.478), Late Xenograft Enriched (FC=1.934, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- The Cancer Genome Atlas (TCGA)
- Genotype-Tissue Expression Project (GTEx)
- Autistic and control brains
- Pre-implantation embryo development