MmuEX0011727 @ mm10
Exon Skipping
Gene
ENSMUSG00000020385 | Clk4
Description
CDC like kinase 4 [Source:MGI Symbol;Acc:MGI:1098551]
Coordinates
chr11:51267171-51272022:+
Coord C1 exon
chr11:51267171-51267331
Coord A exon
chr11:51270537-51270595
Coord C2 exon
chr11:51271800-51272022
Length
59 bp
Sequences
Splice sites
3' ss Seq
GTGTCCCCTCTCTCCCCCAGTGC
3' ss Score
10.73
5' ss Seq
CAGGTAGAG
5' ss Score
7.1
Exon sequences
Seq C1 exon
ATGCGGCATTCCAAACGAACTCACTGTCCTGATTGGGATAGTAGAGAAAGCTGGGGCCATGAAAGCTACAGTGGAAGTCACAAACGCAAGAGAAGGTCTCACAGCAGTACTCAGGAGAACAGGCACTGTAAACCACATCATCAGTTTAAAGACTCGGATTG
Seq A exon
TGCTCCATGTGCATCCCTCTTGAAGCTTCGCACTCTGTTGAAGAGGACACTCATCCCAG
Seq C2 exon
TCACTATTTAGAAGCAAGATGCTTGAATGAGAGAGATTATCGGGACCGGAGATACATTGATGAATACAGAAATGACTACTGCGAAGGATATGTTCCAAGACATTACCATAGAGACGTTGAAAGCACTTACCGGATCCATTGCAGTAAATCCTCAGTCAGGAGCAGGAGAAGCAGCCCTAAGAGAAAGCGTAATAGACCCTGTGCAAGTCATCAGTCGCATTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020385_MULTIEX1-3/3=C1-C2
Average complexity
C1*
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.185 A=0.000 C2=0.319
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTCACAAACGCAAGAGAAGGT
R:
TCAATGTATCTCCGGTCCCGA
Band lengths:
146-205
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.903, FDR=0.138), PC9 No Change at 14 days (FC=1.057, FDR=0.478), Late Xenograft Enriched (FC=1.934, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types