BtaEX0021553 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000010616 | MBD6
Description
methyl-CpG binding domain protein 6 [Source:HGNC Symbol;Acc:HGNC:20445]
Coordinates
chr5:56276101-56277003:-
Coord C1 exon
chr5:56276834-56277003
Coord A exon
chr5:56276506-56276569
Coord C2 exon
chr5:56276101-56276246
Length
64 bp
Sequences
Splice sites
3' ss Seq
CATTGCCTCCCTTCTTGAAGCCA
3' ss Score
6.34
5' ss Seq
AAGGTACAA
5' ss Score
6.82
Exon sequences
Seq C1 exon
GCGGCTGTCCCTCTGCCTCCCCGGGCCCGCCCTGGCCGTCCTGCCAAAAACAAGAGGAGGAAACTGGCCCCATAGCAGCCATACCTGGAGCTGGATCTGACCCTGACTGGGGAGAGCTGAGTGCTGAGCCTTGGGAGCCCCTGCCAGCCACCTGCACCTGTGGACAGTGG
Seq A exon
CCAGCAGGCTCAGTTTACAAACCTGTGAGCTACTGTTGGCTGCTGCCCTCCTTCCCAATGAAAG
Seq C2 exon
AGACGTTGTGACGATGCGACTGAGGATTATGCAACATGGTCCAACCGGAGCGGCCAGCATGACCAGCTGTCCAGGGGCTGCCTCCTGCCTTTTCTTTTGTAAAGACAAGACCCTTGGGAGTTTTAATTCTGTTTTGTACTTGCCCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000010616-'15-16,'15-13,16-16
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
3' UTR
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=NA
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NA
Main Inclusion Isoform:
NA
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TACCTGGAGCTGGATCTGACC
R:
CCGGTTGGACCATGTTGCATA
Band lengths:
137-201
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.577, FDR=0.000), PC9 No Change at 14 days (FC=0.826, FDR=0.019), Late Xenograft Depleted (FC=0.770, FDR=0.004).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development