BtaEX0023572 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000006489 | MYO5A
Description
myosin VA (heavy chain 12, myoxin) [Source:HGNC Symbol;Acc:HGNC:7602]
Coordinates
chr10:58119780-58124805:+
Coord C1 exon
chr10:58119780-58120007
Coord A exon
chr10:58122439-58122447
Coord C2 exon
chr10:58124705-58124805
Length
9 bp
Sequences
Splice sites
3' ss Seq
GCTGACTTGTGATTTTTCAGGAT
3' ss Score
8.61
5' ss Seq
AAGGTAATT
5' ss Score
8.83
Exon sequences
Seq C1 exon
CGTCAAGAACTAGAGTCAGAAAACAAAAAACTGAAAAATGAGCTAAATGAGTTACGAAAGGCCCTCAGTGAGAAAAGTGCCCCTGAGGTGACTGCCCCAGGCGCGCCAGCCTACCGTGTCCTCATGGAGCAGCTGACGTCTGTGAGTGAGGAGCTTGATGTCCGCAAGGAGGAAGTCCTCATCCTGAGGTCTCAGCTGGTGAGCCAGAAGGAAGCCATACAACCCAAG
Seq A exon
GATGACAAG
Seq C2 exon
AACACAATGACAGATTCCACAATACTTTTAGAAGATGTACAGAAAATGAAAGATAAAGGCGAAATAGCACAAGCATATATTGGTTTGAAAGAAACCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006489-MICROEX2-1/2
Average complexity
MIC_M
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.599 A=1.000 C2=0.471
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAGCCAGAAGGAAGCCATAC
R:
TGCTTGTGCTATTTCGCCTTT
Band lengths:
105-114
Functional annotations
There are 2 annotated functions for this event
PMID: 17029413
DLC2 was found to bind as a homodimer to a approximately 15 residue segment (Ile1280-Ile1294) localized between the medial and distal coiled-coil domains of the tail of MYO5A. The binding region contains the three residues coded by the alternatively spliced microexon B (Asp1284-Lys1286). Removal of microexon B eliminates DLC2 binding. Co-localization experiments in a transfected mammalian cell line confirm the finding that exon B is essential for DLC2 binding.
PMID: 16981716
Microexon B constitutes an essential part of the DYNLL2 binding site. A brain-specific isoform in which exon B is skipped can no longer interact with DYNLL2. These data show that alternative splicing of the myosin Va heavy chain controls DYNLL2-myosin Va interaction and that DYNLL2 binding alters the structure of a portion of the myosin's coiled-coil domain. These results suggest that exon B could have a significant impact on the conformation and regulatory folding of native myosin Va, as well as on its interaction with certain cargos.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development