DreEX0048705 @ danRer10
Exon Skipping
Gene
ENSDARG00000061635 | myo5aa
Description
myosin VAa [Source:ZFIN;Acc:ZDB-GENE-041027-2]
Coordinates
chr18:39036144-39037597:+
Coord C1 exon
chr18:39036144-39036371
Coord A exon
chr18:39036506-39036514
Coord C2 exon
chr18:39037497-39037597
Length
9 bp
Sequences
Splice sites
3' ss Seq
TCGTGTTGCATTCATAACAGGAT
3' ss Score
6.52
5' ss Seq
AAGGTATGT
5' ss Score
9.79
Exon sequences
Seq C1 exon
CGTCAGGAGCTGGAGTCTGAGAACAAGAAGCTGAAGCATGATCTGAATCAGATGCGTCAGTCTCTCAGAGGCAGCGTTGAGGCCGGTTCTGGTGCTCCTGGTTCTCCTGCGTACACCGTTCTTCTGGATCAGCTCAATGCCTCCAACGAGGAACTGGAAGTGCGCAAAGAGGAGGTGCTCATCCTGCGCTCACAGCTCGTCAGTCAGAAAGAAGCCATGCAGCACAAG
Seq A exon
GATGAGAAG
Seq C2 exon
ACGACTATGACTGAACCTCTGCCATACATTGAGGATGTGCAGAAGCTCACTGATGCCAAGGAGATCTCACAGGCCTACATGGGCCTCAAAGACACCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061635-MICROEX2
Average complexity
MIC-S
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.658 A=1.000 C2=0.727
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSDART00000159834fB4065
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAAAGAAGCCATGCAGCACA
R:
AGGCCTGTGAGATCTCCTTGG
Band lengths:
99-108
Functional annotations
There are 1 annotated functions for this event
PMID: 16981716
Microexon B constitutes an essential part of the DYNLL2 binding site. A brain-specific isoform in which exon B is skipped can no longer interact with DYNLL2. These data show that alternative splicing of the myosin Va heavy chain controls DYNLL2-myosin Va interaction and that DYNLL2 binding alters the structure of a portion of the myosin's coiled-coil domain. These results suggest that exon B could have a significant impact on the conformation and regulatory folding of native myosin Va, as well as on its interaction with certain cargos.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]