BtaEX6077705 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000010057 | GZMB
Description
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) [Source:HGNC Symbol;Acc:HGNC:4709]
Coordinates
chr21:35265363-35266587:-
Coord C1 exon
chr21:35266440-35266587
Coord A exon
chr21:35265818-35265953
Coord C2 exon
chr21:35265363-35265620
Length
136 bp
Sequences
Splice sites
3' ss Seq
CTGCTCTGGTCTCTGTGCAGCTC
3' ss Score
9.7
5' ss Seq
AAGGTAAAC
5' ss Score
8.14
Exon sequences
Seq C1 exon
GGGAGATCATCGGGGGCCATGAAGCCAAGCCCCACTCCCGCCCCTACATGGCATATCTTCAGTACTGGAATCAGGATGTCCAGAGTAGGTGCGGTGGGTTCCTGGTTCGACAGGACTTCGTGCTGACAGCCGCTCACTGCAACGGAAG
Seq A exon
CTCAATCAAAGTCACCCTGGGGGCCCACAACATCAAACAGCAGGAGAGGACCCAGCAGGTCATCAGGGTGAGAAGAGCCATCAGCCACCCTGACTATAATCCTAAGAACTTCTCCAACGACATCATGTTATTAAAG
Seq C2 exon
CTGGAGAGAAAGGCCAAGCAGACATCAGCTGTGAAGCCCCTTAGTCTGCCCAGGGCCAAGGCCCGGGTGAAGCCAGGACAGACGTGCAGCGTGGCCGGCTGGGGGAGGGACTCCACGGACACCTACGCTGACACACTACAGGAGGTAAAGCTGATCGTGCAGGAGGATCAAAAGTGTGAGGCCTACTTACGCAACTTTTATAACCGCGCCATCCAGCTGTGTGTGGGGGACCCAAAGACAAAGAAAGCTTCCTTTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000010057-'1-3,'1-2,2-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.080 A=0.043 C2=0.058
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=PU(21.5=94.0)
A:
PF0008921=Trypsin=FE(20.5=100)
C2:
PF0008921=Trypsin=FE(38.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGTTCGACAGGACTTCGTG
R:
GGCGCGGTTATAAAAGTTGCG
Band lengths:
257-393
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]