BtaEX6087177 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000037794 | MYH4
Description
myosin, heavy chain 4, skeletal muscle [Source:HGNC Symbol;Acc:HGNC:7574]
Coordinates
chr19:30087651-30089199:-
Coord C1 exon
chr19:30088810-30089199
Coord A exon
chr19:30088180-30088306
Coord C2 exon
chr19:30087651-30087769
Length
127 bp
Sequences
Splice sites
3' ss Seq
TTTTCTACTTCGCACCGCAGGGA
3' ss Score
8.37
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
Exon sequences
Seq C1 exon
GCCCGCATCGAGGAGCTGGAGGAGGAGATCGAGGCTGAGCGGGCCTCCCGCGCCAAAGCAGAGAAGCAGCGCTCAGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGCGGCTAGAAGAAGCCGGCGGGGCCACTTCCGCCCAGATTGAGATGAACAAGAAGCGGGAGGCCGAGTTCCAGAAGATGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGCGGCCGCTCTTCGCAAGAAGCACGCGGACAGTGTGGCCGAGCTGGGGGAGCAGATTGACAACCTGCAGAGGGTCAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGACGACCTGGCCAGCAACATGGAGACTGTCTCCAAAGCCAAA
Seq A exon
GGAAACCTCGAGAAAATGTGCCGTACACTAGAGGACCAACTGAGTGAAGTGAAAAGTAAGGAAGAGGAGCACCAGCGCCTAATCAATGAACTGTCAGCCCAGAAGGCACATCTACACACAGAGTCAG
Seq C2 exon
GTGAATTTTCACGACAGCTAGAAGAGAAAGATGCTCTGGTGTCTCAGCTATCCCGAGGCAAACAAGCATTTACACAACAGATTGAGGAATTAAAGAGGCAGCTAGAAGAGGAGACTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000037794-'24-25,'24-24,25-25=AN
Average complexity
A_S
Mappability confidence:
88%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.923 A=1.000 C2=0.550
Domain overlap (PFAM):
C1:
PF087025=Fib_alpha=PD(16.2=14.6),PF0157614=Myosin_tail_1=FE(15.0=100)
A:
PF0157614=Myosin_tail_1=FE(4.9=100)
C2:
PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=PU(11.8=87.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGAGGAGGCCACCCTG
R:
CGGGATAGCTGAGACACCAGA
Band lengths:
250-377
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]