MmuEX0030367 @ mm10
Exon Skipping
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67253154-67254576:+
Coord C1 exon
chr11:67253154-67253543
Coord A exon
chr11:67254000-67254126
Coord C2 exon
chr11:67254458-67254576
Length
127 bp
Sequences
Splice sites
3' ss Seq
TCCTTTACTTCCCATCACAGGGG
3' ss Score
8.74
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
Exon sequences
Seq C1 exon
GCCCGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCTAAAGCAGAGAAGCAGCGCTCTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCTGGCGGAGCCACTTCAGCCCAGATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTGCAGCATGAAGCCACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGATCTCGCTAGTAACATGGAGACAGTCTCCAAAGCCAAG
Seq A exon
GGGAACCTTGAGAAGATGTGCCGCACCCTGGAGGACCAACTGAGTGAAGTGAAGACCAAGGAGGAGGAGCAGCAGCGCCTGATCAATGAACTGTCAACCCAGAAGGCACGCTTGCACACAGAGTCAG
Seq C2 exon
GCGAGTTTTCCCGACAGCTGGATGAAAAAGACGCTATGGTTTCTCAGCTTTCCCGAGGCAAACAAGCATTTACACAACAGATTGAGGAACTAAAGAGGCAGCTAGAGGAAGAGTCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-'44-41,'44-35,46-41=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.923 A=1.000 C2=0.825
Domain overlap (PFAM):
C1:
PF087025=Fib_alpha=PD(14.7=13.1),PF0157614=Myosin_tail_1=FE(15.0=100)
A:
PF0157614=Myosin_tail_1=FE(4.9=100),PF059116=DUF869=PU(30.3=62.8),PF0003816=Filament=PU(4.2=30.2)
C2:
PF0157614=Myosin_tail_1=FE(4.5=100),PF059116=DUF869=FE(43.8=100),PF0003816=Filament=FE(12.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCAGATCGACAACCTGCAG
R:
TTTCGTCCTCCACTGGGCTAC
Band lengths:
258-385
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types