BtaINT0013192 @ bosTau6
Intron Retention
Gene
ENSBTAG00000037449 | ACSM5
Description
acyl-CoA synthetase medium-chain family member 5 [Source:HGNC Symbol;Acc:HGNC:26060]
Coordinates
chr25:18231211-18231523:+
Coord C1 exon
chr25:18231211-18231338
Coord A exon
chr25:18231339-18231423
Coord C2 exon
chr25:18231424-18231523
Length
85 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAAG
5' ss Score
1.99
3' ss Seq
AGTTTGTTTTTCTCTTCCAGCTA
3' ss Score
9.61
Exon sequences
Seq C1 exon
GACAACCCTGAGAAGACAGCAGCATCAGAACGAGGGGACTTCTACATCACAGGGGACCGAGCCCACATGGATAAGGATGGCTACTTTTGGTTCATGGGAAGAAATGATGACGTGATCAATTCCTCAAG
Seq A exon
GTCAAGTTGTCTATACTTTCCTTCTTCCTTTGAAGCTTCATAGGACAGAGGAGGACCCTTGAAAGAGTTTGTTTTTCTCTTCCAG
Seq C2 exon
CTACCGGATCGGGCCTGTTGAGGTGGAGAGTGCACTTGCTGAGCACCCAGCTGTCCTGGAGTCTGCTGTGGTCAGCAGCCCGGACCCCATTAGAGGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000037449:ENSBTAT00000054846:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.163 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=PD(9.9=95.3)
A:
NA
C2:
PF131931=AMP-binding_C=PU(32.1=76.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAACCCTGAGAAGACAGCAGC
R:
CTCAGCAAGTGCACTCTCCAC
Band lengths:
169-254
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]