Special

MmuINT0010285 @ mm10

Intron Retention

Gene
Description
acyl-CoA synthetase medium-chain family member 5 [Source:MGI Symbol;Acc:MGI:2444086]
Coordinates
chr7:119540605-119540910:+
Coord C1 exon
chr7:119540605-119540732
Coord A exon
chr7:119540733-119540810
Coord C2 exon
chr7:119540811-119540910
Length
78 bp
Sequences
Splice sites
5' ss Seq
AAGGTCAGG
5' ss Score
6.23
3' ss Seq
AGTTTGCTTTTCTCTTCCAGCTA
3' ss Score
8.99
Exon sequences
Seq C1 exon
GACAATCCCGAGAAGACAGCAGCATCAGAGCAAGGAGACTTTTACATCACTGGGGACAGAGCACATATGGACGAGGATGGCTACTTTTGGTTCTTGGGGAGAAATGATGATGTAATCAACTCTTCAAG
Seq A exon
GTCAGGGTAGCTCTTCCTCTCCTTGAGCACCCATAGGGGAAGGGAAGCCATGTGAAAGAGTTTGCTTTTCTCTTCCAG
Seq C2 exon
CTACCGGATTGGTCCTGTTGAAGTAGAGAGTGCACTTGCTGAGCATCCAGCTGTCCTGGAGTCTGCTGTGGTCAGCAGCCCAGACCCCATCCGGGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030972:ENSMUST00000066465:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.186 A=NA C2=0.044
Domain overlap (PFAM):

C1:
PF0050123=AMP-binding=PD(9.9=95.3)
A:
NA
C2:
PF131931=AMP-binding_C=PU(32.1=76.5)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
Zebrafish
(danRer10)
ALTERNATIVE
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACAGCAGCATCAGAGCAAG
R:
AGACTCCAGGACAGCTGGATG
Band lengths:
179-257
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types